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Title: | FANCD2 protects genome stability by recruiting RNA processing enzymes to resolve R-loops during mild replication stress |
Authors: | Okamoto, Yusuke Abe, Masako Itaya, Akiko Tomida, Junya Ishiai, Masamichi Takaori‐Kondo, Akifumi Taoka, Masato Isobe, Toshiaki Takata, Minoru https://orcid.org/0000-0002-4926-3675 (unconfirmed) |
Author's alias: | 板谷, 亜希子 石合, 正道 高折, 晃史 髙田, 穣 |
Keywords: | DDX47 FANCD2 hnRNP U replication stress R‐loops |
Issue Date: | 9-Jan-2019 |
Publisher: | Wiley |
Journal title: | The FEBS Journal |
Volume: | 286 |
Issue: | 1 |
Start page: | 139 |
End page: | 150 |
Abstract: | R‐loops, which consist of DNA : RNA hybrids and displaced single‐strand DNA, are a major threat to genome stability. We have previously reported that a key Fanconi anemia protein, FANCD2, accumulates on large fragile genes during mild replication stress in a manner depending on R‐loops. In this study, we found that FANCD2 suppresses R‐loop levels. Furthermore, we identified FANCD2 interactions with RNA processing factors, including hnRNP U and DDX47. Our data suggest that FANCD2, which accumulates with R‐loops in chromatin, recruits these factors and thereby promotes efficient processing of long RNA transcripts. This may lead to a reduction in transcription–replication collisions, as detected by PLA between PCNA and RNA Polymerase II, and hence, lowered R‐loop levels. We propose that this mechanism might contribute to maintenance of genome stability during mild replication stress. |
Rights: | This is the peer reviewed version of the following article: Okamoto, Y. , Abe, M. , Itaya, A. , Tomida, J. , Ishiai, M. , Takaori‐Kondo, A. , Taoka, M. , Isobe, T. and Takata, M. (2019), FANCD2 protects genome stability by recruiting RNA processing enzymes to resolve R‐loops during mild replication stress. FEBS J, 286: 139-150, which has been published in final form at https://doi.org/10.1111/febs.14700. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. The full-text file will be made open to the public on 9 January 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/241711 |
DOI(Published Version): | 10.1111/febs.14700 |
PubMed ID: | 30431240 |
Appears in Collections: | Journal Articles |
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