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dc.contributor.author | Masunaga, Shin-ichiro | en |
dc.contributor.author | Uzawa, Akiko | en |
dc.contributor.author | Hirayama, Ryoichi | en |
dc.contributor.author | Matsumoto, Yoshitaka | en |
dc.contributor.author | Sakurai, Yoshinori | en |
dc.contributor.author | Tanaka, Hiroki | en |
dc.contributor.author | Tano, Keizo | en |
dc.contributor.author | Sanada, Yu | en |
dc.contributor.author | Suzuki, Minoru | en |
dc.contributor.author | Maruhashi, Akira | en |
dc.contributor.author | Ono, Koji | en |
dc.contributor.alternative | 増永, 慎一郎 | ja |
dc.contributor.alternative | 真田, 悠生 | ja |
dc.date.accessioned | 2019-06-12T06:37:19Z | - |
dc.date.available | 2019-06-12T06:37:19Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1920-4531 | - |
dc.identifier.issn | 1920-454X | - |
dc.identifier.uri | http://hdl.handle.net/2433/241735 | - |
dc.description.abstract | Background: The aim of the study was to clarify the effect of p53 status of tumor cells on radiosensitivity of solid tumors following accelerated carbon-ion beam irradiation compared with γ-rays or reactor neutron beams, referring to the response of intratumor quiescent (Q) cells. Methods: Human head and neck squamous cell carcinoma cells transfected with mutant TP53 (SAS/mp53) or with neo vector (SAS/neo) were injected subcutaneously into hind legs of nude mice. Tumor-bearing mice received 5-bromo-2’-deoxyuridine (BrdU) continuously to label all intratumor proliferating (P) cells. They received γ-rays or accelerated carbon-ion beams at a high or reduced dose-rate. Other tumor-bearing mice received reactor thermal or epithermal neutrons at a reduced dose-rate. Immediately or 9 hours after the high dose-rate irradiation (HDRI), or immediately after the reduced dose-rate irradiation (RDRI), the tumor cells were isolated and incubated with a cytokinesis blocker, and the micronucleus (MN) frequency in cells without BrdU labeling (Q cells) was determined using immunofluorescence staining for BrdU. Results: The difference in radiosensitivity between the total (P + Q) and Q cells after γ-ray irradiation was markedly reduced with reactor neutron beams or carbon-ion beams, especially with a higher linear energy transfer (LET) value. Following γ-ray irradiation, SAS/neo tumor cells, especially intratumor Q cells, showed a marked reduction in sensitivity due to the recovery from radiation-induced damage, compared with the total or Q cells within SAS/mp53 tumors that showed little repair capacity. In both total and Q cells within both SAS/neo and SAS/mp53 tumors, carbon-ion beam irradiation, especially with a higher LET, showed little recovery capacity through leaving an interval between HDRI and the assay or decreasing the dose-rate. The recovery from radiation-induced damage after γ-ray irradiation was a p53-dependent event, but little recovery was found after carbon-ion beam irradiation. With RDRI, the radiosensitivity to reactor thermal and epithermal neutron beams was slightly higher than that to carbon-ion beams. Conclusion: For tumor control, including intratumor Q-cell control, accelerated carbon-ion beams, especially with a higher LET, and reactor thermal and epithermal neutron beams were very useful for suppressing the recovery from radiation-induced damage irrespective of p53 status of tumor cells. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elmer Press, Inc. | en |
dc.rights | © The authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en |
dc.subject | p53 status | en |
dc.subject | Quiescent cell | en |
dc.subject | Carbon-ion beams | en |
dc.subject | Reactor neutron beams | en |
dc.subject | γ-rays | en |
dc.title | The Effect of p53 Status of Tumor Cells on Radiosensitivity of Irradiated Tumors With Carbon-Ion Beams Compared With γ-Rays or Reactor Neutron Beams | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | World Journal of Oncology | - |
dc.identifier.volume | 6 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 398 | - |
dc.identifier.epage | 409 | - |
dc.relation.doi | 10.14740/wjon941w | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 28983338 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 26670556 | - |
datacite.awardNumber | 15H04295 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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