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Title: Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock
Authors: Uehara, Takahiro N.
Mizutani, Yoshiyuki
Kuwata, Keiko
Hirota, Tsuyoshi
Sato, Ayato
Mizoi, Junya
Takao, Saori
Matsuo, Hiromi
Suzuki, Takamasa
Ito, Shogo
Saito, Ami N.
Nishiwaki-Ohkawa, Taeko
Yamaguchi-Shinozaki, Kazuko
Yoshimura, Takashi
Kay, Steve A.
Itami, Kenichiro
Kinoshita, Toshinori
Yamaguchi, Junichiro
Nakamichi, Norihito
Author's alias: 上原, 貴大
桑田, 啓子
廣田, 毅
佐藤, 綾人
溝井, 順哉
鈴木, 孝征
伊藤, 照悟
大川, 妙子
篠崎, 和子
吉村, 崇
伊丹, 健一郎
木下, 俊則
山口, 潤一郎
中道, 範人
Keywords: circadian clock
Arabidopsis
small molecule
posttranslational regulation
Issue Date: 4-Jun-2019
Publisher: Proceedings of the National Academy of Sciences
Journal title: Proceedings of the National Academy of Sciences
Volume: 116
Issue: 23
Start page: 11528
End page: 11536
Abstract: The circadian clock provides organisms with the ability to adapt to daily and seasonal cycles. Eukaryotic clocks mostly rely on lineage-specific transcriptional-translational feedback loops (TTFLs). Posttranslational modifications are also crucial for clock functions in fungi and animals, but the posttranslational modifications that affect the plant clock are less understood. Here, using chemical biology strategies, we show that the Arabidopsis CASEIN KINASE 1 LIKE (CKL) family is involved in posttranslational modification in the plant clock. Chemical screening demonstrated that an animal CDC7/CDK9 inhibitor, PHA767491, lengthens the Arabidopsis circadian period. Affinity proteomics using a chemical probe revealed that PHA767491 binds to and inhibits multiple CKL proteins, rather than CDC7/CDK9 homologs. Simultaneous knockdown of Arabidopsis CKL-encoding genes lengthened the circadian period. CKL4 phosphorylated transcriptional repressors PSEUDO-RESPONSE REGULATOR 5 (PRR5) and TIMING OF CAB EXPRESSION 1 (TOC1) in the TTFL. PHA767491 treatment resulted in accumulation of PRR5 and TOC1, accompanied by decreasing expression of PRR5- and TOC1-target genes. A prr5 toc1 double mutant was hyposensitive to PHA767491-induced period lengthening. Together, our results reveal posttranslational modification of transcriptional repressors in plant clock TTFL by CK1 family proteins, which also modulate nonplant circadian clocks.
Rights: © 2019 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
URI: http://hdl.handle.net/2433/241774
DOI(Published Version): 10.1073/pnas.1903357116
PubMed ID: 31097584
Appears in Collections:Journal Articles

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