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タイトル: | Casein kinase 1 family regulates PRR5 and TOC1 in the Arabidopsis circadian clock |
著者: | Uehara, Takahiro N. Mizutani, Yoshiyuki Kuwata, Keiko Hirota, Tsuyoshi Sato, Ayato Mizoi, Junya Takao, Saori Matsuo, Hiromi Suzuki, Takamasa Ito, Shogo https://orcid.org/0000-0002-8434-6652 (unconfirmed) Saito, Ami N. Nishiwaki-Ohkawa, Taeko Yamaguchi-Shinozaki, Kazuko Yoshimura, Takashi Kay, Steve A. Itami, Kenichiro Kinoshita, Toshinori Yamaguchi, Junichiro Nakamichi, Norihito |
著者名の別形: | 上原, 貴大 桑田, 啓子 廣田, 毅 佐藤, 綾人 溝井, 順哉 鈴木, 孝征 伊藤, 照悟 大川, 妙子 篠崎, 和子 吉村, 崇 伊丹, 健一郎 木下, 俊則 山口, 潤一郎 中道, 範人 |
キーワード: | circadian clock Arabidopsis small molecule posttranslational regulation |
発行日: | 4-Jun-2019 |
出版者: | Proceedings of the National Academy of Sciences |
誌名: | Proceedings of the National Academy of Sciences |
巻: | 116 |
号: | 23 |
開始ページ: | 11528 |
終了ページ: | 11536 |
抄録: | The circadian clock provides organisms with the ability to adapt to daily and seasonal cycles. Eukaryotic clocks mostly rely on lineage-specific transcriptional-translational feedback loops (TTFLs). Posttranslational modifications are also crucial for clock functions in fungi and animals, but the posttranslational modifications that affect the plant clock are less understood. Here, using chemical biology strategies, we show that the Arabidopsis CASEIN KINASE 1 LIKE (CKL) family is involved in posttranslational modification in the plant clock. Chemical screening demonstrated that an animal CDC7/CDK9 inhibitor, PHA767491, lengthens the Arabidopsis circadian period. Affinity proteomics using a chemical probe revealed that PHA767491 binds to and inhibits multiple CKL proteins, rather than CDC7/CDK9 homologs. Simultaneous knockdown of Arabidopsis CKL-encoding genes lengthened the circadian period. CKL4 phosphorylated transcriptional repressors PSEUDO-RESPONSE REGULATOR 5 (PRR5) and TIMING OF CAB EXPRESSION 1 (TOC1) in the TTFL. PHA767491 treatment resulted in accumulation of PRR5 and TOC1, accompanied by decreasing expression of PRR5- and TOC1-target genes. A prr5 toc1 double mutant was hyposensitive to PHA767491-induced period lengthening. Together, our results reveal posttranslational modification of transcriptional repressors in plant clock TTFL by CK1 family proteins, which also modulate nonplant circadian clocks. |
著作権等: | © 2019 the Author(s). Published by PNAS. This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND). |
URI: | http://hdl.handle.net/2433/241774 |
DOI(出版社版): | 10.1073/pnas.1903357116 |
PubMed ID: | 31097584 |
出現コレクション: | 学術雑誌掲載論文等 |
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