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Title: The effects of short-term hypoxia on human mesenchymal stem cell proliferation, viability and p16(INK4A) mRNA expression: Investigation using a simple hypoxic culture system with a deoxidizing agent
Authors: Ito, Akira
Aoyama, Tomoki
Yoshizawa, Makoto
Nagai, Momoko
Tajino, Junichi
Yamaguchi, Shoki
Iijima, Hirotaka
Zhang, Xiangkai
Kuroki, Hiroshi
Author's alias: 伊藤, 明良
青山, 朋樹
太治野, 純一
山口, 将希
飯島, 弘貴
黒木, 裕士
Keywords: Hypoxia
Mesenchymal stem cell
p16INK4A
Deoxidizing agent
Transportation
Issue Date: 30-May-2015
Publisher: GN Corporation Co. Ltd.
Journal title: Journal of stem cells & regenerative medicine
Volume: 11
Issue: 1
Start page: 25
End page: 31
Abstract: A hypoxic environment is thought to be important for the maintenance of stemness and suppressing cell senescence, in stem cells. Therefore, a hypoxic condition is induced during cell expansion and/or induction of intended differentiation. However, the induction of these conditions requires a specially equipped hypoxia chamber and expensive gas mixtures, which are expensive and space-consuming. Owing to these restrictions, appropriate hypoxic conditions cannot be provided during cell transportation, which is increasingly required for regenerative medicine. Hence, a simple and economical culture system is required. The purpose of this study was to investigate the effects of short-term hypoxic conditions on human mesenchymal stem cell (MSC) proliferation, viability, and senescence, utilizing the CulturePal system (CulturePal-Zero and CulturePal-Five), a novel and simple hypoxic culture system with a built-in deoxidizing agent. The O₂ concentration in the CulturePal-Zero was observed to reduce to <0.1% within 1 h, and to 5% within 24 h in the CulturePal-Five system. Cell proliferation under these hypoxic conditions showed a sharp increase at 5% O₂ concentration, and no noticeable cell death was observed even at severe hypoxic conditions (<0.1% O₂) up to 72h. The p16INK4A (cell senescence marker) mRNA expression was retained under hypoxic conditions up to 72h, but it was up-regulated under normoxic conditions. Interestingly, the p16INK4A expression altered proportionately to the O₂ concentration. These results indicated that the short-term hypoxic condition, at an approximate O₂ concentration of 5%, would be suitable for promoting cell proliferation and repressing cell senescence, without aggravating the MSC viability. Therefore, the CulturePal systems may be suitable for providing an appropriate hypoxic condition in stem cell research and transportation.
Rights: This is an open-access article distributed under the terms of the Creative Commons 3.0 Unported (CC BY 3.0) Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
URI: http://hdl.handle.net/2433/241788
PubMed ID: 26195892
Related Link: https://www.pubstemcell.com/monthly/011010300005.htm
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