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タイトル: | Transcriptome analysis of a thermophilic and hydrogenogenic carboxydotroph Carboxydothermus pertinax |
著者: | Fukuyama, Yuto Omae, Kimiho Yoshida, Takashi https://orcid.org/0000-0002-5257-0617 (unconfirmed) Sako, Yoshihiko |
著者名の別形: | 福山, 宥斗 大前, 公保 吉田, 天士 左子, 芳彦 |
キーワード: | Hydrogenogenic carboxydotroph Carbon monoxide dehydrogenase RNA sequencing Carboxydothermus pertinax |
発行日: | Jul-2019 |
出版者: | Springer Nature |
誌名: | Extremophiles |
巻: | 23 |
号: | 4 |
開始ページ: | 389 |
終了ページ: | 398 |
抄録: | A thermophilic and hydrogenogenic carboxydotroph, Carboxydothermus pertinax, performs hydrogenogenic CO metabolism in which CODH-II couples with distally encoded ECH. To enhance our knowledge of its hydrogenogenic CO metabolism, we performed whole transcriptome analysis of C. pertinax grown under 100% CO or 100% N₂ using RNA sequencing. Of the 2577 genes, 36 and 64 genes were differentially expressed genes (DEGs) with false discovery rate adjusted P value < 0.05 when grown under 100% CO or 100% N₂, respectively. Most of the DEGs were components of 23 gene clusters, suggesting switch between metabolisms via intensive expression changes in a relatively low number of gene clusters. Of the 9 significantly expressed gene clusters under 100% CO, CODH-II and ECH gene clusters were found. Only the ECH gene cluster was regulated by the CO-responsive transcriptional factor CooA, suggesting that others were separately regulated in the same transcriptional cascade as the ECH gene cluster. Of the 14 significantly expressed gene clusters under 100% N₂, ferrous iron transport gene cluster involved in anaerobic respiration and prophage region were found. Considering that the expression of the temperate phage was strictly repressed under 100% CO, hydrogenogenic CO metabolism might be stable for C. pertinax. |
著作権等: | © The Author(s) 2019. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
URI: | http://hdl.handle.net/2433/243263 |
DOI(出版社版): | 10.1007/s00792-019-01091-x |
PubMed ID: | 30941583 |
出現コレクション: | 学術雑誌掲載論文等 |
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