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dc.contributor.authorChowdhury, Pratiti H.
dc.contributor.authorKitamura, Gaku
dc.contributor.authorHonda, Akiko
dc.contributor.authorSawahara, Takahiro
dc.contributor.authorHayashi, Tomohiro
dc.contributor.authorFukushima, Wataru
dc.contributor.authorKudo, Hitomi
dc.contributor.authorIto, Sho
dc.contributor.authorYoshida, Seiichi
dc.contributor.authorIchinose, Takamichi
dc.contributor.authorUeda, Kayo
dc.contributor.authorTakano, Hirohisa
dc.contributor.alternative本田, 晶子
dc.contributor.alternative上田, 佳代
dc.contributor.alternative高野, 裕久
dc.date.accessioned2019-08-29T03:55:52Z-
dc.date.available2019-08-29T03:55:52Z-
dc.date.issued2017-9
dc.identifier.issn1520-4081
dc.identifier.issn1522-7278
dc.identifier.urihttp://hdl.handle.net/2433/243826-
dc.description.abstractParticulate matter with aerodynamic diameter ≤2.5 μm (PM₂.₅) is generally composed of carbon nuclei associated with various organic carbons, metals, ions and biological materials. Among these components, polyaromatic hydrocarbons (PAHs) such as benzo(a)pyrene (BaP) and quinones have detrimental effects on airway epithelial cells and immunodisrupting effects, which leads to the exacerbation of respiratory allergies. The effects of PAHs and the carbon nuclei, separately as well as in combination, remain to be established. We investigated the effects of BaP, 9, 10‐phenanthroquinone (9, 10‐PQ), and 1, 2‐napthoquinone (1, 2‐NQ) and their combined effects with heated diesel exhaust particle (H‐DEP) as carbon nuclei of typical PM₂.₅. We exposed human airway epithelial cells (BEAS‐2B), murine bone marrow‐derived antigen‐presenting cells (APCs), and murine splenocytes to BaP, 9, 10‐PQ, or 1, 2‐NQ in the presence and absence of H‐DEP. Several important inflammatory cytokines and cell surface molecules were measured. PAHs alone did not have apparent cytotoxic effects on BEAS‐2B, whereas combined exposure with H‐DEP induced noticeable detrimental effects which mainly reflected the action of H‐DEP itself. BaP increased CD86 expression as an APC surface molecule regardless of the presence or absence of H‐DEP. None of the BaP, 9, 10‐PQ, or 1, 2‐NQ exposure alone or their combined exposure with H‐DEP resulted in any significant activation of splenocytes. These results suggest that PAHs and carbon nuclei show additive effects, and that BaP with the carbon nuclei may contribute to exacerbations of allergic respiratory diseases including asthma by PM₂.₅, especially via antigen‐presenting cell activation.
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherWiley
dc.rightsThis is the peer reviewed version of the following article: 'Environmental Toxicology' 32(9) 2172-2181, which has been published in final form at https://doi.org/10.1002/tox.22430. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
dc.rightsThe full-text file will be made open to the public on 16 August 2018 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
dc.subjectantigen‐presenting cell
dc.subjectimmune response
dc.subjectparticulate matter
dc.subjectpolyaromatic hydrocarbon
dc.subjectrespiratory health
dc.titleSynergistic effect of carbon nuclei and polyaromatic hydrocarbons on respiratory and immune responses
dc.type.niitypeJournal Article
dc.identifier.jtitleEnvironmental Toxicology
dc.identifier.volume32
dc.identifier.issue9
dc.identifier.spage2172
dc.identifier.epage2181
dc.relation.doi10.1002/tox.22430
dc.textversionauthor
dc.addressEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University
dc.addressEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University
dc.addressEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University
dc.addressEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University
dc.addressEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University
dc.addressEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University
dc.addressEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University
dc.addressEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University
dc.addressDepartment of Health Sciences, Oita University of Nursing and Health Sciences
dc.addressDepartment of Health Sciences, Oita University of Nursing and Health Sciences
dc.addressEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University
dc.addressEnvironmental Health Division, Department of Environmental Engineering, Graduate School of Engineering, Kyoto University
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