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Title: | Novel hybrid three-dimensional artificial liver using human induced pluripotent stem cells and a rat decellularized liver scaffold |
Authors: | Minami, Takahito Ishii, Takamichi ![]() ![]() ![]() Yasuchika, Kentaro Fukumitsu, Ken ![]() ![]() Ogiso, Satoshi ![]() ![]() Miyauchi, Yuya Kojima, Hidenobu Kawai, Takayuki Yamaoka, Ryoya Oshima, Yu Kawamoto, Hiroshi ![]() ![]() ![]() Kotaka, Maki Yasuda, Katsutaro Osafune, Kenji ![]() ![]() Uemoto, Shinji ![]() ![]() |
Author's alias: | 石井, 隆道 小木曾, 聡 小髙, 真希 長船, 健二 上本, 伸二 |
Keywords: | Decellularized liver Recellularization Human iPSC Hepatocyte Artificial liver |
Issue Date: | Jun-2019 |
Publisher: | Elsevier BV |
Journal title: | Regenerative therapy |
Volume: | 10 |
Start page: | 127 |
End page: | 133 |
Abstract: | Introduction: Liver transplantation is currently the only curative therapy for end-stage liver failure; however, establishment of alternative treatments is required owing to the serious donor organ shortage. Here, we propose a novel model of hybrid three-dimensional artificial livers using both human induced pluripotent stem cells (hiPSCs) and a rat decellularized liver serving as a scaffold. Methods: Rat liver harvesting and decellularization were performed as reported in our previous studies. The decellularized liver scaffold was recellularized with hiPSC-derived hepatocyte-like cells (hiPSC-HLCs) through the biliary duct. The recellularized liver graft was continuously perfused with the culture medium using a pump at a flow rate of 0.5 mL/min in a standard CO₂(5%) cell incubator at 37 °C. Results: After 48 h of continuous perfusion culture, the hiPSC-HLCs of the recellularized liver distributed into the parenchymal space. Furthermore, the recellularized liver expressed the albumin (ALB) and CYP3A4 genes, and secreted human ALB into the culture medium. Conclusion: Novel hybrid artificial livers using hiPSCs and rat decellularized liver scaffolds were successfully generated, which possessed human hepatic functions. |
Rights: | © 2019, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
URI: | http://hdl.handle.net/2433/243894 |
DOI(Published Version): | 10.1016/j.reth.2019.03.002 |
PubMed ID: | 31032388 |
Appears in Collections: | Journal Articles |

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