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Title: Novel hybrid three-dimensional artificial liver using human induced pluripotent stem cells and a rat decellularized liver scaffold
Authors: Minami, Takahito
Ishii, Takamichi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-7461-9653 (unconfirmed)
Yasuchika, Kentaro
Fukumitsu, Ken  kyouindb  KAKEN_id
Ogiso, Satoshi
Miyauchi, Yuya
Kojima, Hidenobu
Kawai, Takayuki
Yamaoka, Ryoya
Oshima, Yu
Kawamoto, Hiroshi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-7670-9900 (unconfirmed)
Kotaka, Maki
Yasuda, Katsutaro
Osafune, Kenji  kyouindb  KAKEN_id
Uemoto, Shinji  kyouindb  KAKEN_id
Author's alias: 石井, 隆道
小木曾, 聡
小髙, 真希
長船, 健二
上本, 伸二
Keywords: Decellularized liver
Recellularization
Human iPSC
Hepatocyte
Artificial liver
Issue Date: Jun-2019
Publisher: Elsevier BV
Journal title: Regenerative therapy
Volume: 10
Start page: 127
End page: 133
Abstract: Introduction: Liver transplantation is currently the only curative therapy for end-stage liver failure; however, establishment of alternative treatments is required owing to the serious donor organ shortage. Here, we propose a novel model of hybrid three-dimensional artificial livers using both human induced pluripotent stem cells (hiPSCs) and a rat decellularized liver serving as a scaffold. Methods: Rat liver harvesting and decellularization were performed as reported in our previous studies. The decellularized liver scaffold was recellularized with hiPSC-derived hepatocyte-like cells (hiPSC-HLCs) through the biliary duct. The recellularized liver graft was continuously perfused with the culture medium using a pump at a flow rate of 0.5 mL/min in a standard CO₂(5%) cell incubator at 37 °C. Results: After 48 h of continuous perfusion culture, the hiPSC-HLCs of the recellularized liver distributed into the parenchymal space. Furthermore, the recellularized liver expressed the albumin (ALB) and CYP3A4 genes, and secreted human ALB into the culture medium. Conclusion: Novel hybrid artificial livers using hiPSCs and rat decellularized liver scaffolds were successfully generated, which possessed human hepatic functions.
Rights: © 2019, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
URI: http://hdl.handle.net/2433/243894
DOI(Published Version): 10.1016/j.reth.2019.03.002
PubMed ID: 31032388
Appears in Collections:Journal Articles

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