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Title: Development and characterization of EST-SSR markers for Carex angustisquama (Cyperaceae), an extremophyte in solfatara fields
Authors: Nagasawa, Koki
Setoguchi, Hiroaki  kyouindb  KAKEN_id
Maki, Masayuki
Goto, Hayato
Fukushima, Keitaro
Isagi, Yuji  kyouindb  KAKEN_id  orcid (unconfirmed)
Sakaguchi, Shota  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 長澤, 耕樹
瀬戸口, 浩彰
福島, 慶太郎
井鷺, 裕司
阪口, 翔太
Keywords: Carex angustisquama
Carex doenitzii
expressed sequence tag–simple sequence repeat (EST‐SSR) markers
Issue Date: Oct-2018
Publisher: Wiley
Journal title: Applications in plant sciences
Volume: 6
Issue: 10
Thesis number: e01185
Abstract: Premise of the Study: Expressed sequence tag–simple sequence repeat (EST‐SSR) markers were developed for Carex angustisquama (Cyperaceae) to investigate the evolutionary history of this plant that is endemic to solfatara fields in northern Japan. Methods and Results: Using RNA‐Seq data generated by the Illumina HiSeq 2000, 20 EST‐SSR markers were developed. Polymorphisms were assessed in C. angustisquama and the closely related species C. doenitzii and C. podogyna. In C. angustisquama, many loci were monomorphic within populations; the average number of alleles ranged from one to five, and levels of expected heterozygosity ranged from 0.000 to 0.580, while all markers were polymorphic in a population of C. doenitzii. This indicates that low genetic polymorphism of C. angustisquama is likely due to the species’ population dynamics, rather than to null alleles at the developed markers. Conclusions: These markers will be used to assess genetic diversity and structure and to investigate evolutionary history in future studies of C. angustisquama and related species.
Rights: © 2018 Nagasawa et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
DOI(Published Version): 10.1002/aps3.1185
PubMed ID: 30386711
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