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タイトル: | Time-Resolved Diffusion Detection with Microstopped Flow System |
著者: | Nakasone, Yusuke Takaramoto, Shunki Terazima, Masahide https://orcid.org/0000-0001-6828-479X (unconfirmed) |
著者名の別形: | 中曽根, 祐介 寺嶋, 正秀 |
発行日: | 17-Sep-2019 |
出版者: | NLM (Medline) |
誌名: | Analytical chemistry |
巻: | 91 |
号: | 18 |
開始ページ: | 11987 |
終了ページ: | 11993 |
抄録: | The transient grating (TG) method is a powerful technique for monitoring the time dependence of the diffusion coefficient during photochemical reactions. However, the applications of this technique have been limited to photochemical reactions. Here, a microstopped flow (μ-SF) system is developed to expand the technique’s applicability. The constructed μ-SF system can be used for a solution with a total volume as small as 3 μL, and mixing times for absorption and diffusion measurements were determined to be 400 μs and 100 ms, respectively. To demonstrate this system with the TG method, an acid-induced denaturation of a photosensor protein, phototropin LOV2 domain with a linker, was studied from the viewpoint of the reactivity. This system can be used not only for time-resolved diffusion measurement but also for conventional absorption or fluorescence detection methods. In particular, this system has a great advantage for a target solution in that only a very small amount is needed. |
著作権等: | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Analytical chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.analchem.9b02897. The full-text file will be made open to the public on 23 August 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/244208 |
DOI(出版社版): | 10.1021/acs.analchem.9b02897 |
PubMed ID: | 31442029 |
出現コレクション: | 学術雑誌掲載論文等 |
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