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Title: Development of a regulatable low-temperature protein expression system using the psychrotrophic bacterium, Shewanella livingstonensis Ac10, as the host
Authors: Kawai, Soichiro
Kawamoto, Jun
Ogawa, Takuya  kyouindb  KAKEN_id
Kurihara, Tatsuo  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 河合, 総一郎
川本, 純
小川, 拓哉
栗原, 達夫
Keywords: Trp promoter
heterologous protein production
regulatable gene expression
Issue Date: 2019
Publisher: NLM (Medline)
Journal title: Bioscience, biotechnology, and biochemistry
Volume: 83
Issue: 11
Start page: 2153
End page: 2162
Abstract: A low-temperature protein expression system is useful for the production of thermolabile proteins. We previously developed a system that enables constitutive protein production at low temperatures, using the psychrotrophic bacterium Shewanella livingstonensis Ac10 as the host. To increase the utility of this system, in the present study, we introduced a repressible promoter of the trp operon of this bacterium into the system. When ß-lactamase was produced under the control of this promoter at 18°C and 4°C, the yields were 75 and 33 mg/L-culture, respectively, in the absence of L-Trp, and the yields were decreased by 72% and 77%, respectively, in the presence of L-Trp. We also found that 3-indoleacrylic acid, a competitive inhibitor of the Escherichia coli trp repressor, increased the expression of the reporter gene. This repressible gene expression system would be useful for regulatable recombinant protein production at low temperatures.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, biotechnology, and biochemistry on 11 July 2019, available online:
The full-text file will be made open to the public on 11 July 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1080/09168451.2019.1638754
PubMed ID: 31291825
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