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|Title:||Development of a regulatable low-temperature protein expression system using the psychrotrophic bacterium, Shewanella livingstonensis Ac10, as the host|
Kurihara, Tatsuo https://orcid.org/0000-0002-7777-1378 (unconfirmed)
|Author's alias:||河合, 総一郎|
heterologous protein production
regulatable gene expression
|Journal title:||Bioscience, biotechnology, and biochemistry|
|Abstract:||A low-temperature protein expression system is useful for the production of thermolabile proteins. We previously developed a system that enables constitutive protein production at low temperatures, using the psychrotrophic bacterium Shewanella livingstonensis Ac10 as the host. To increase the utility of this system, in the present study, we introduced a repressible promoter of the trp operon of this bacterium into the system. When ß-lactamase was produced under the control of this promoter at 18°C and 4°C, the yields were 75 and 33 mg/L-culture, respectively, in the absence of L-Trp, and the yields were decreased by 72% and 77%, respectively, in the presence of L-Trp. We also found that 3-indoleacrylic acid, a competitive inhibitor of the Escherichia coli trp repressor, increased the expression of the reporter gene. This repressible gene expression system would be useful for regulatable recombinant protein production at low temperatures.|
|Rights:||This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, biotechnology, and biochemistry on 11 July 2019, available online: http://www.tandfonline.com/10.1080/09168451.2019.1638754.|
The full-text file will be made open to the public on 11 July 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version.
|Appears in Collections:||Journal Articles|
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