このアイテムのアクセス数: 698
このアイテムのファイル:
| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| 978-1-4939-9740-4_16.pdf | 75.86 kB | Adobe PDF | 見る/開く |
| タイトル: | Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal |
| 著者: | Yamano, Takashi    https://orcid.org/0000-0001-6578-8095 (unconfirmed)Fukuzawa, Hideya |
| 著者名の別形: | 山野, 隆志 福澤, 秀哉 |
| キーワード: | Chlamydomonas reinhardtii Electroporation Photosynthesis Protist Square electric pulse |
| 発行日: | 2020 |
| 出版者: | Springer US |
| 誌名: | Methods in Molecular Biology |
| 巻: | 2050 |
| 開始ページ: | 155 |
| 終了ページ: | 161 |
| 抄録: | The green alga Chlamydomonas reinhardtii has been widely used to study many biological processes, including photosynthesis, flagellar motility, sexual reproduction, metabolism, and genetics. Here, we describe a step-by-step protocol of rapid and efficient transformation method for wild type cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to the transformation of other industrially useful algae including diatom by optimizing the electric conditions. |
| 記述: | Yamano T., Fukuzawa H. (2020) Transformation of the Model Microalga Chlamydomonas reinhardtii Without Cell-Wall Removal. In: Li S., Chang L., Teissie J. (eds) Electroporation Protocols. Methods in Molecular Biology, vol 2050. Humana, New York, NY |
| 著作権等: | This is a post-peer-review, pre-copyedit version of an article published in Methods in Molecular Biology. The final authenticated version is available online at: https://doi.org/10.1007/978-1-4939-9740-4_16. The full-text file will be made open to the public on 30 August 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
| URI: | http://hdl.handle.net/2433/244795 |
| DOI(出版社版): | 10.1007/978-1-4939-9740-4_16 |
| PubMed ID: | 31468489 |
| 出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。