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Title: Modified nucleobase-specific gene regulation using engineered transcription activator-like effectors
Authors: Tsuji, Shogo
Imanishi, Miki
Author's alias: 今西, 未来
Keywords: DNA binding
DNA methylation
Epigenetics
Gene manipulation
Gene targeting
Issue Date: Jul-2019
Publisher: Elsevier B.V.
Journal title: Advanced Drug Delivery Reviews
Volume: 147
Start page: 59
End page: 65
Abstract: Epigenetic modification, as typified by cytosine methylation, is a key aspect of gene regulation that affects many biological processes. However, the biological roles of individual methylated cytosines are poorly understood. Sequence-specific DNA recognition tools can be used to investigate the roles of individual instances of DNA methylation. Transcription activator-like effectors (TALEs), which are DNA-binding proteins, are promising candidate tools with designable sequence specificity and sensitivity to DNA methylation. In this review, we describe the bases of DNA recognition of TALEs, including methylated cytosine recognition, and the applications of TALEs for the study of methylated DNA. In addition, we discuss TALE-based epigenome editing and oxidized methylated cytosine recognition.
Rights: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
The full-text file will be made open to the public on 1 July 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/244858
DOI(Published Version): 10.1016/j.addr.2019.08.011
PubMed ID: 31513826
Appears in Collections:Journal Articles

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