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DCフィールド | 値 | 言語 |
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dc.contributor.author | Honda, Yoichi | en |
dc.contributor.author | Tanigawa, Eiji | en |
dc.contributor.author | Tsukihara, Takahisa | en |
dc.contributor.author | Nguyen, Dong Xuan | en |
dc.contributor.author | Kawabe, Harunori | en |
dc.contributor.author | Sakatoku, Naofumi | en |
dc.contributor.author | Watari, Junko | en |
dc.contributor.author | Sato, Hideaki | en |
dc.contributor.author | Yano, Shigekazu | en |
dc.contributor.author | Tachiki, Takashi | en |
dc.contributor.author | Irie, Toshikazu | en |
dc.contributor.author | Watanabe, Takahito | en |
dc.contributor.author | Watanabe, Takashi | en |
dc.contributor.alternative | 本田, 与一 | ja |
dc.contributor.alternative | 渡邊, 崇人 | ja |
dc.contributor.alternative | 渡邊, 隆司 | ja |
dc.date.accessioned | 2020-01-17T02:18:33Z | - |
dc.date.available | 2020-01-17T02:18:33Z | - |
dc.date.issued | 2019-06-24 | - |
dc.identifier.issn | 2191-0855 | - |
dc.identifier.uri | http://hdl.handle.net/2433/245407 | - |
dc.description.abstract | A genetic transformation system was developed for the selective white rot basidiomycete Ceriporiopsis subvermispora using a modified protocol with polyethylene glycol and CaCl₂ treatment of the protoplasts and plasmids harboring recombinant hygromycin phosphotransferase (hph) driven by a homologous promoter. During repeated transfer on fresh potato dextrose agar plates containing 100 µg/ml hygromycin B, most transformants lost drug resistance, while the remaining isolates showed stable resistance over five transfers. No drug-resistant colonies appeared in control experiments without DNA or using a promoter-less derivative of the plasmid, indicating that a transient expression of the recombinant hph was driven by the promoter sequence in these unstable drug-resistant transformants. Southern blot analysis of the stable transformants revealed random integration of the plasmid DNA fragment in the chromosome at different copy numbers. This transformation system yielding mostly transient transformants was successfully used for promoter assay experiments, and only a 141-bp fragment was found to be essential for the basic promoter function of glyceraldehyde dehydrogenase gene (gpd) in this fungus. Subsequent mutational analyses suggested that a TATAA sequence is important for the basic promoter function of gpd gene. The promoter assay system will enable the functional analysis of gene expression control sequences quickly and easily, mostly in the absence of undesirable effects from differences in copy number and chromosomal position of an integrated reporter gene among stable transformants. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Springer Science and Business Media LLC | en |
dc.rights | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | en |
dc.subject | Basidiomycete | en |
dc.subject | Genetic transformation | en |
dc.subject | Transient gene expression | en |
dc.subject | Promoter assay | en |
dc.subject | TATAA sequence | en |
dc.title | Stable and transient transformation, and a promoter assay in the selective lignin-degrading fungus, Ceriporiopsis subvermispora | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | AMB Express | en |
dc.identifier.volume | 9 | - |
dc.relation.doi | 10.1186/s13568-019-0818-1 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 92 | - |
dc.address | Graduate School of Agriculture, Kyoto University・Research Institute for Sustainable Humanosphere, Kyoto University | en |
dc.address | Research Institute for Sustainable Humanosphere, Kyoto University | en |
dc.address | Research Institute for Sustainable Humanosphere, Kyoto University | en |
dc.address | Graduate School of Agriculture, Kyoto University・Biotechnology Center of Ho Chi Minh City | en |
dc.address | Research Institute for Sustainable Humanosphere, Kyoto University | en |
dc.address | Research Institute for Sustainable Humanosphere, Kyoto University | en |
dc.address | Research Institute for Sustainable Humanosphere, Kyoto University | en |
dc.address | Research Institute for Sustainable Humanosphere, Kyoto University | en |
dc.address | Graduate School of Life Sciences, Ritsumeikan University・Graduate School of Science and Engineering, Yamagata University | en |
dc.address | Biotechnology Center of Ho Chi Minh City | en |
dc.address | Environmental Science Graduate School, The University of Shiga Prefecture | en |
dc.address | Research Institute for Sustainable Humanosphere, Kyoto University | en |
dc.address | Research Institute for Sustainable Humanosphere, Kyoto University | en |
dc.identifier.pmid | 31236750 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | JP11760124 | - |
datacite.awardNumber | JP13760130 | - |
datacite.awardNumber | JP21580402 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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