ダウンロード数: 132
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
aps3.11275.pdf | 285.05 kB | Adobe PDF | 見る/開く |
タイトル: | Development and characterization of EST-SSR markers for Saxifraga fortunei var. incisolobata (Saxifragaceae) |
著者: | Magota, Kana Takahashi, Daiki Setoguchi, Hiroaki |
著者名の別形: | 孫田, 佳奈 高橋, 大樹 瀬戸口, 浩彰 |
キーワード: | ecological polymorphisms expressed sequence tag–simple sequence repeat (EST-SSR) markers Saxifraga fortunei Saxifragaceae transcriptome |
発行日: | Jul-2019 |
出版者: | Wiley |
誌名: | Applications in Plant Sciences |
巻: | 7 |
号: | 7 |
論文番号: | e11275 |
抄録: | Premise: Saxifraga fortunei (Saxifragaceae) includes several infraspecific taxa that are ecologically and morphologically distinct. To investigate the evolutionary history of phenotypic polymorphisms in this species, we developed expressed sequence tag–simple sequence repeat (EST‐SSR) markers for S. fortunei. Methods and Results: We developed 26 polymorphic markers based on transcriptome data obtained from Illumina HiSeq 2000. Within three populations of S. fortunei var. incisolobata, the number of alleles ranged from four to 25, and the levels of observed and expected heterozygosity ranged from 0.200 to 0.847 and from 0.209 to 0.930, respectively. Furthermore, all 26 loci showed transferability for S. fortunei var. obtusocuneata and S. fortunei var. suwoensis, and 18 loci were also successfully amplified in S. acerifolia. Conclusions: These newly developed EST‐SSR markers will prove useful to infer the evolutionary history of S. fortunei var. incisolobata and its relatives in population genetic studies. |
著作権等: | © 2019 Magota et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/245654 |
DOI(出版社版): | 10.1002/aps3.11275 |
PubMed ID: | 31346507 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。