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Title: Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum
Authors: Gotoh, Aina
Katoh, Toshihiko  kyouindb  KAKEN_id
Sakanaka, Mikiyasu
Ling, Yiwei
Yamada, Chihaya
Asakuma, Sadaki
Urashima, Tadasu
Tomabechi, Yusuke
Katayama-Ikegami, Ayako
Kurihara, Shin
Yamamoto, Kenji
Harata, Gaku
He, Fang
Hirose, Junko
Kitaoka, Motomitsu
Okuda, Shujiro
Katayama, Takane  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-4009-7874 (unconfirmed)
Author's alias: 後藤, 愛那
加藤, 紀彦
阪中, 幹祥
山田, 千早
朝隈, 貞樹
浦島, 匡
苫米地, 祐輔
片山(池上), 礼子
栗原, 新
山本, 憲二
原田, 岳
廣瀬, 潤子
北岡, 本光
奥田, 修二郎
片山, 高嶺
Keywords: Bacterial physiology
Microbial ecology
Issue Date: 18-Sep-2018
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 8
Thesis number: 13958
Abstract: Gut microbiota of breast-fed infants are generally rich in bifidobacteria. Recent studies show that infant gut-associated bifidobacteria can assimilate human milk oligosaccharides (HMOs) specifically among the gut microbes. Nonetheless, little is known about how bifidobacterial-rich communities are shaped in the gut. Interestingly, HMOs assimilation ability is not related to the dominance of each species. Bifidobacterium longum susbp. longum and Bifidobacterium breve are commonly found as the dominant species in infant stools; however, they show limited HMOs assimilation ability in vitro. In contrast, avid in vitro HMOs consumers, Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis, are less abundant in infant stools. In this study, we observed altruistic behaviour by B. bifidum when incubated in HMOs-containing faecal cultures. Four B. bifidum strains, all of which contained complete sets of HMO-degrading genes, commonly left HMOs degradants unconsumed during in vitro growth. These strains stimulated the growth of other Bifidobacterium species when added to faecal cultures supplemented with HMOs, thereby increasing the prevalence of bifidobacteria in faecal communities. Enhanced HMOs consumption by B. bifidum-supplemented cultures was also observed. We also determined the complete genome sequences of B. bifidum strains JCM7004 and TMC3115. Our results suggest B. bifidum-mediated cross-feeding of HMOs degradants within bifidobacterial communities.
Rights: © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/246545
DOI(Published Version): 10.1038/s41598-018-32080-3
PubMed ID: 30228375
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