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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s41598-018-31370-0.pdf | 3.12 MB | Adobe PDF | 見る/開く |
| タイトル: | X-ray structure analysis of bacteriorhodopsin at 1.3 Å resolution |
| 著者: | Hasegawa, Nagayuki Jonotsuka, Hideyuki Miki, Kunio Takeda, Kazuki    https://orcid.org/0000-0002-4094-6816 (unconfirmed) |
| 著者名の別形: | 長谷川, 修之 城塚, 秀之 三木, 邦夫 竹田, 一旗 |
| キーワード: | Molecular biophysics X-ray crystallography |
| 発行日: | 3-Sep-2018 |
| 出版者: | Springer Nature |
| 誌名: | Scientific Reports |
| 巻: | 8 |
| 論文番号: | 13123 |
| 抄録: | Bacteriorhodopsin (bR) of Halobacterium salinarum is a membrane protein that acts as a light-driven proton pump. bR and its homologues have recently been utilized in optogenetics and other applications. Although the structures of those have been reported so far, the resolutions are not sufficient for elucidation of the intrinsic structural features critical to the color tuning and ion pumping properties. Here we report the accurate crystallographic analysis of bR in the ground state. The influence of X-rays was suppressed by collecting the data under a low irradiation dose at 15 K. Consequently, individual atoms could be separately observed in the electron density map at better than 1.3 Å resolution. Residues from Thr5 to Ala233 were continuously constructed in the model. The twist of the retinal polyene was determined to be different from those in the previous models. Two conformations were observed for the proton release region. We discuss the meaning of these fine structural features. |
| 著作権等: | © The Author(s) 2018. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| URI: | http://hdl.handle.net/2433/246548 |
| DOI(出版社版): | 10.1038/s41598-018-31370-0 |
| PubMed ID: | 30177765 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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