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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s41598-017-08259-5.pdf | 2.57 MB | Adobe PDF | 見る/開く |
| タイトル: | Measurement of caveolin-1 densities in the cell membrane for quantification of caveolar deformation after exposure to hypotonic membrane tension |
| 著者: | Tachikawa, Masashi  Morone, Nobuhiro Senju, Yosuke Sugiura, Tadao Hanawa-Suetsugu, Kyoko Mochizuki, Atsushi   https://orcid.org/0000-0002-7714-1755 (unconfirmed)Suetsugu, Shiro |
| 著者名の別形: | 立川, 正志 諸根, 信弘 千住, 洋介 杉浦, 忠男 塙(末次), 京子 望月, 敦史 末次, 志郎 |
| キーワード: | Caveolae Super-resolution microscopy |
| 発行日: | 10-Aug-2017 |
| 出版者: | Springer Nature |
| 誌名: | Scientific Reports |
| 巻: | 7 |
| 論文番号: | 7794 |
| 抄録: | Caveolae are abundant flask-shaped invaginations of plasma membranes that buffer membrane tension through their deformation. Few quantitative studies on the deformation of caveolae have been reported. Each caveola contains approximately 150 caveolin-1 proteins. In this study, we estimated the extent of caveolar deformation by measuring the density of caveolin-1 projected onto a two-dimensional (2D) plane. The caveolin-1 in a flattened caveola is assumed to have approximately one-quarter of the density of the caveolin-1 in a flask-shaped caveola. The proportion of one-quarter-density caveolin-1 increased after increasing the tension of the plasma membrane through hypo-osmotic treatment. The one-quarter-density caveolin-1 was soluble in detergent and formed a continuous population with the caveolin-1 in the caveolae of cells under isotonic culture. The distinct, dispersed lower-density caveolin-1 was soluble in detergent and increased after the application of tension, suggesting that the hypo-osmotic tension induced the dispersion of caveolin-1 from the caveolae, possibly through flattened caveolar intermediates. |
| 著作権等: | © The Author(s) 2017. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| URI: | http://hdl.handle.net/2433/249985 |
| DOI(出版社版): | 10.1038/s41598-017-08259-5 |
| PubMed ID: | 28798329 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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