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Title: Identification and functional characterisation of N-linked glycosylation of the orphan G protein-coupled receptor Gpr176
Authors: Wang, Tianyu
Nakagawa, Shumpei
Miyake, Takahito  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-4356-5883 (unconfirmed)
Setsu, Genzui
Kunisue, Sumihiro
Goto, Kaoru
Hirasawa, Akira  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-5692-805X (unconfirmed)
Okamura, Hitoshi
Yamaguchi, Yoshiaki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-4126-542X (unconfirmed)
Doi, Masao  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-6264-9217 (unconfirmed)
Author's alias: 三宅, 崇仁
平澤, 明
岡村, 均
山口, 賀章
土居, 雅夫
Keywords: Circadian rhythms and sleep
Glycosylation
Hormone receptors
Mutation
Post-translational modifications
Issue Date: 10-Mar-2020
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 10
Thesis number: 4429
Abstract: G-protein-coupled receptors (GPCRs) are important drug targets with diverse therapeutic applications. However, there are still more than a hundred orphan GPCRs, whose protein functions and biochemical features remain unidentified. Gpr176 encodes a class-A orphan GPCR that has a role in circadian clock regulation in mouse hypothalamus and is also implicated in human breast cancer transcriptional response. Here we show that Gpr176 is N-glycosylated. Peptide-N-glycosidase treatment of mouse hypothalamus extracts revealed that endogenous Gpr176 undergoes N-glycosylation. Using a heterologous expression system, we show that N-glycosylation occurs at four conserved asparagine residues in the N-terminal region of Gpr176. Deficient N-glycosylation due to mutation of these residues reduced the protein expression of Gpr176. At the molecular function level, Gpr176 has constitutive, agonist-independent activity that leads to reduced cAMP synthesis. Although deficient N-glycosylation did not compromise this intrinsic activity, the resultant reduction in protein expression was accompanied by attenuation of cAMP-repressive activity in the cells. We also demonstrate that human GPR176 is N-glycosylated. Importantly, missense variations in the conserved N-glycosylation sites of human GPR176 (rs1473415441; rs761894953) affected N-glycosylation and thereby attenuated protein expression and cAMP-repressive activity in the cells. We show that N-glycosylation is a prerequisite for the efficient protein expression of functional Gpr176/GPR176.
Rights: © The Author(s) 2020. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/250077
DOI(Published Version): 10.1038/s41598-020-61370-y
PubMed ID: 32157140
Appears in Collections:Journal Articles

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