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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s13568-020-00983-y.pdf | 4.34 MB | Adobe PDF | 見る/開く |
| タイトル: | Evaluation of the yeast surface display system for screening of functional nanobodies |
| 著者: | Kajiwara, Kaho Aoki, Wataru   https://orcid.org/0000-0002-3118-9390 (unconfirmed)Ueda, Mitsuyoshi  |
| 著者名の別形: | 梶原, 果穂 青木, 航 植田, 充美 |
| キーワード: | Cell surface display Screening platform Protein engineering Library screening Nanobody Saccharomyces cerevisiae |
| 発行日: | 16-Mar-2020 |
| 出版者: | Springer Science and Business Media LLC |
| 誌名: | AMB Express |
| 巻: | 10 |
| 論文番号: | 51 |
| 抄録: | Yeast surface display is a powerful technology used to isolate and engineer proteins to improve their activity, specificity, and stability. In this method, gene expression is regulated by promoters, and secretion efficiency is affected by secretion signals. Furthermore, both the accessibility and activity of the displayed proteins are affected by the length of anchor proteins. The ideal promoter, secretion signal, and anchor protein combination depend on the proteins of interest. In this study, we optimized a yeast surface display suitable for nanobody evaluation. We designed five display systems that used different combinations of promoters, secretion signals, and anchor proteins. Anti-hen egg-white lysozyme nanobody was used as the model nanobody. The amount of nanobodies displayed on yeast cells, the number of antigens bound to the displayed nanobodies, and the display efficiency were quantified. Overall, we improved the yeast display system for nanobody engineering and proposed its optimization. |
| 著作権等: | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| URI: | http://hdl.handle.net/2433/250108 |
| DOI(出版社版): | 10.1186/s13568-020-00983-y |
| PubMed ID: | 32180052 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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