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Title: Methanol production by reversed methylotrophy constructed in Escherichia coli
Authors: Takeya, Tomoyuki
Yamakita, Miyabi
Hayashi, Daisuke
Fujisawa, Kento
Sakai, Yasuyoshi  kyouindb  KAKEN_id
Yurimoto, Hiroya  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 竹谷, 友之
由里本, 博也
Keywords: Methanol dehydrogenase
3-hexulose-6-phosphate synthase
ribulose monophosphate pathway
Bacillus methanolicus
Mycobacterium gastri
Issue Date: 3-May-2020
Publisher: Taylor & Francis Group
Journal title: Bioscience, Biotechnology, and Biochemistry
Volume: 84
Issue: 5
Start page: 1062
End page: 1068
Abstract: We constructed a reversed methylotrophic pathway that produces methanol, a promising feedstock for production of useful compounds, from fructose 6-phosphate (F6P), which can be supplied by catabolism of biomass-derived sugars including glucose, by a synthetic biology approach. Using Escherichia coli as an expression host, we heterologously expressed genes encoding methanol utilization enzymes from methylotrophic bacteria, i.e. the NAD⁺-dependent methanol dehydrogenase (MDH) from Bacillus methanolicus S1 and an artificial fusion enzyme of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase from Mycobacterium gastri MB19 (HPS-PHI). We confirmed that these enzymes can catalyze reverse reactions of methanol oxidation and formaldehyde fixation. The engineered E. coli strain co-expressing MDH and HPS-PHI genes produced methanol in resting cell reactions not only from F6P but also from glucose. We successfully conferred reversed methylotrophy to E. coli and our results provide a proof-of-concept for biological methanol production from biomass-derived sugar compounds.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in 'Bioscience, biotechnology, and biochemistry' on 3 May 2020, available online:
The full-text file will be made open to the public on 16 January 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving'
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1080/09168451.2020.1715202
PubMed ID: 31942827
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