ダウンロード数: 115
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
13416979.2020.1744232.pdf | 134.53 kB | Adobe PDF | 見る/開く |
タイトル: | Development and characterization of nuclear microsatellite markers in Aphananthe aspera (Thunb.) Planch. (Cannabaceae) |
著者: | Sakagami, Taishi Sakaguchi, Shota https://orcid.org/0000-0002-6180-1747 (unconfirmed) Isagi, Yuji https://orcid.org/0000-0002-9777-076X (unconfirmed) Setoguchi, Hiroaki |
著者名の別形: | 阪口, 翔太 井鷺, 裕司 瀬戸口, 浩彰 |
キーワード: | Aphananthe aspera Cannabaceae genetic diversity microsatellite markers |
発行日: | Mar-2020 |
出版者: | Taylor & Francis |
誌名: | Journal of Forest Research |
巻: | 25 |
号: | 2 |
開始ページ: | 120 |
終了ページ: | 123 |
抄録: | Nuclear microsatellite markers were developed for Aphananthe aspera (Thunb.) Planch. (Cannabaceae), a deciduous canopy tree species distributed in East Asia, to evaluate the genetic diversity and genetic structure of A. aspera populations in remnant forest fragments in urbanized areas of Japan. A total of 94 primer pairs were designed based on genomic sequence data. Of the 25 primer pairs which showed clear microsatellite peaks, 20 pairs showed allelic polymorphisms in 57 individuals collected from two distant populations. The length of PCR products ranged from 120 to 482 bp, and expected heterozygosity for the 20 microsatellite markers ranged from 0.017 to 0.768. These newly developed simple sequence repeat markers will be used in population genetic studies of A. aspera to evaluate genetic diversity and the extent of genetic isolation of the fragmented populations in urban areas. |
著作権等: | This is an Accepted Manuscript of an article published by Taylor & Francis in Journal of Forest Research on 24 March 2020, available online: http://www.tandfonline.com/10.1080/13416979.2020.1744232. The full-text file will be made open to the public on 24 March 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/251018 |
DOI(出版社版): | 10.1080/13416979.2020.1744232 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。