ダウンロード数: 194
このアイテムのファイル:
| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s42003-020-1011-4.pdf | 973.77 kB | Adobe PDF | 見る/開く |
| タイトル: | Integral approach to biomacromolecular structure by analytical-ultracentrifugation and small-angle scattering |
| 著者: | Morishima, Ken   https://orcid.org/0000-0003-1091-3850 (unconfirmed)Okuda, Aya  https://orcid.org/0000-0002-6222-8364 (unconfirmed)Inoue, Rintaro Sato, Nobuhiro https://orcid.org/0000-0002-6960-6567 (unconfirmed)Miyamoto, Yosuke Urade, Reiko https://orcid.org/0000-0002-9232-6176 (unconfirmed)Yagi-Utsumi, Maho Kato, Koichi Hirano, Rina Kujirai, Tomoya Kurumizaka, Hitoshi Sugiyama, Masaaki |
| 著者名の別形: | 守島, 健 奥田, 綾 井上, 倫太郎 佐藤, 信浩 宮本, 洋佑 裏出, 令子 矢木, 真穂 加藤, 晃一 平野, 里奈 鯨井, 智也 胡桃坂, 仁志 杉山, 正明 |
| キーワード: | SAXS |
| 発行日: | 8-Jun-2020 |
| 出版者: | Springer Nature |
| 誌名: | Communications Biology |
| 巻: | 3 |
| 論文番号: | 294 |
| 抄録: | Currently, a sample for small-angle scattering (SAS) is usually highly purified and looks monodispersed: The Guinier plot of its SAS intensity shows a fine straight line. However, it could include the slight aggregates which make the experimental SAS profile different from the monodispersed one. A concerted method with analytical-ultracentrifugation (AUC) and SAS, named as AUC-SAS, offers the precise scattering intensity of a concerned biomacromolecule in solution even with aggregates as well that of a complex under an association-dissociation equilibrium. AUC-SAS overcomes an aggregation problem which has been an obstacle for SAS analysis and, furthermore, has a potential to lead to a structural analysis for a general multi-component system. |
| 記述: | 溶液中の蛋白質構造を正確に評価するための新規解析法を開発 --構造評価の妨げとなる凝集の影響を実験データから除去--. 京都大学プレスリリース. 2020-06-09. |
| 著作権等: | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| URI: | http://hdl.handle.net/2433/251196 |
| DOI(出版社版): | 10.1038/s42003-020-1011-4 |
| PubMed ID: | 32513995 |
| 関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2020-06-09-0 |
| 出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。