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タイトル: | Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon) |
著者: | Tani, Tetsuya Eitsuka, Takahiro Katayama, Masafumi Nagamine, Takashi Nakaya, Yumiko Suzuki, Hajime Kiyono, Tohru Nakagawa, Kiyotaka Inoue-Murayama, Miho Onuma, Manabu Fukuda, Tomokazu |
著者名の別形: | 谷, 哲弥 永塚, 貴弘 片山, 雅史 長嶺, 隆 中谷, 裕美子 鈴木, 創 清野, 透 仲川, 清隆 村山, 美穂 大沼, 学 福田, 智一 |
発行日: | 26-Aug-2019 |
出版者: | Public Library of Science (PLoS) |
誌名: | PLOS ONE |
巻: | 14 |
号: | 8 |
論文番号: | e0221364 |
抄録: | The Bonin flying fox (Pteropus pselaphon) is one of the most critically endangered species of animals. The number of this species is estimated to be around 150; being classified at the top rank in the list by International Union of Animal Conservation. Our group previously showed that expression of CDK4, CYCLIN D1, and telomerase reverse transcriptase (TERT) efficiently induce immortalization of human, bovine, swine, monkey, and buffalo-derived cells. In this manuscript, we successfully established the primary cells from Bonin flying fox. We introduced CDK4, CYCLIN D1, and TERT into the primary cells. The established cells showed efficient expression of introduced genes at the protein level. Furthermore, the established cells were free from senescence, indicating it reached to immortalization. Moreover, we showed that interspecies somatic cell nuclear transfer of Bonin flying fox derived cell into bovine embryo allowed the development of the embryo to 8 cell stages. Our established cell has the potential to contribute to species conservation. |
記述: | Correction -- 29 May 2020: Tani T, Eitsuka T, Katayama M, Nagamine T, Nakaya Y, et al. (2020) Correction: Establishment of immortalized primary cell from the critically endangered Bonin flying fox (Pteropus pselaphon). PLOS ONE 15(5): e0234054. |
著作権等: | © 2019 Tani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/252353 |
DOI(出版社版): | 10.1371/journal.pone.0221364 |
PubMed ID: | 31449544 |
関連リンク: | https://doi.org/10.1371/journal.pone.0234054 |
出現コレクション: | 学術雑誌掲載論文等 |
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