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Title: Mesenchymal stromal cells in bone marrow express adiponectin and are efficiently targeted by an adiponectin promoter-driven Cre transgene
Authors: Mukohira, Hisa
Hara, Takahiro  kyouindb  KAKEN_id  orcid (unconfirmed)
Abe, Shinya
Tani-Ichi, Shizue
Sehara-Fujisawa, Atsuko
Nagasawa, Takashi
Tobe, Kazuyuki
Ikuta, Koichi  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 原, 崇裕
阿部, 真也
谷一, 靖江
生田, 宏一
Keywords: adipocyte
bone marrow
stromal cells
Issue Date: Nov-2019
Publisher: Oxford University Press (OUP)
Journal title: International immunology
Volume: 31
Issue: 11
Start page: 729
End page: 742
Abstract: Stromal cells in bone marrow (BM) constitute a specific microenvironment supporting the development and maintenance of hematopoietic cells. Adiponectin is a cytokine secreted by adipocytes. Besides its anti-diabetic and anti-atherogenic roles, adiponectin reportedly regulates the development and function of hematopoietic cells in BM. However, it remains unclear whether mesenchymal stromal cells in BM express adiponectin. Here, we show that PDGFRβ+VCAM-1+ stromal cells express adiponectin. Lineage tracing revealed that a majority of PDGFRβ+VCAM-1+ cells were targeted by an adiponectin promoter-driven Cre (Adipoq-Cre) transgene. Additionally, the Adipoq-Cre transgene targets a minority of osteoblasts at a younger age but larger populations are targeted at an older age. Furthermore, the Adipoq-Cre transgene targets almost all CXCL12-abundant reticular (CAR) cells and most of the stromal cells targeted by the Adipoq-Cre transgene are CAR cells. Finally, deletion of interleukin-7 (IL-7) by the Adipoq-Cre transgene resulted in severe impairment of B lymphopoiesis in BM. These results demonstrate that PDGFRβ+VCAM-1+ stromal cells in BM express adiponectin and are targeted by the Adipoq-Cre transgene, suggesting a broader specificity of the Adipoq-Cre transgene.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in International immunology following peer review. The version of record Hisa Mukohira, Takahiro Hara, Shinya Abe, Shizue Tani-ichi, Atsuko Sehara-Fujisawa, Takashi Nagasawa, Kazuyuki Tobe, Koichi Ikuta, Mesenchymal stromal cells in bone marrow express adiponectin and are efficiently targeted by an adiponectin promoter-driven Cre transgene, International Immunology, Volume 31, Issue 11, November 2019, Pages 729–742 is available online at:
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1093/intimm/dxz042
PubMed ID: 31094421
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