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タイトル: | Mesenchymal stromal cells in bone marrow express adiponectin and are efficiently targeted by an adiponectin promoter-driven Cre transgene |
著者: | Mukohira, Hisa Hara, Takahiro https://orcid.org/0000-0002-3845-8434 (unconfirmed) Abe, Shinya Tani-Ichi, Shizue Sehara-Fujisawa, Atsuko Nagasawa, Takashi Tobe, Kazuyuki Ikuta, Koichi https://orcid.org/0000-0003-1319-1021 (unconfirmed) |
著者名の別形: | 原, 崇裕 阿部, 真也 谷一, 靖江 生田, 宏一 |
キーワード: | adipocyte adiponectin bone marrow CXCL12 IL-7 stromal cells |
発行日: | Nov-2019 |
出版者: | Oxford University Press (OUP) |
誌名: | International immunology |
巻: | 31 |
号: | 11 |
開始ページ: | 729 |
終了ページ: | 742 |
抄録: | Stromal cells in bone marrow (BM) constitute a specific microenvironment supporting the development and maintenance of hematopoietic cells. Adiponectin is a cytokine secreted by adipocytes. Besides its anti-diabetic and anti-atherogenic roles, adiponectin reportedly regulates the development and function of hematopoietic cells in BM. However, it remains unclear whether mesenchymal stromal cells in BM express adiponectin. Here, we show that PDGFRβ+VCAM-1+ stromal cells express adiponectin. Lineage tracing revealed that a majority of PDGFRβ+VCAM-1+ cells were targeted by an adiponectin promoter-driven Cre (Adipoq-Cre) transgene. Additionally, the Adipoq-Cre transgene targets a minority of osteoblasts at a younger age but larger populations are targeted at an older age. Furthermore, the Adipoq-Cre transgene targets almost all CXCL12-abundant reticular (CAR) cells and most of the stromal cells targeted by the Adipoq-Cre transgene are CAR cells. Finally, deletion of interleukin-7 (IL-7) by the Adipoq-Cre transgene resulted in severe impairment of B lymphopoiesis in BM. These results demonstrate that PDGFRβ+VCAM-1+ stromal cells in BM express adiponectin and are targeted by the Adipoq-Cre transgene, suggesting a broader specificity of the Adipoq-Cre transgene. |
著作権等: | This is a pre-copyedited, author-produced version of an article accepted for publication in International immunology following peer review. The version of record Hisa Mukohira, Takahiro Hara, Shinya Abe, Shizue Tani-ichi, Atsuko Sehara-Fujisawa, Takashi Nagasawa, Kazuyuki Tobe, Koichi Ikuta, Mesenchymal stromal cells in bone marrow express adiponectin and are efficiently targeted by an adiponectin promoter-driven Cre transgene, International Immunology, Volume 31, Issue 11, November 2019, Pages 729–742 is available online at: https://doi.org/10.1093/intimm/dxz042. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/253542 |
DOI(出版社版): | 10.1093/intimm/dxz042 |
PubMed ID: | 31094421 |
出現コレクション: | 学術雑誌掲載論文等 |
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