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タイトル: | CRISPR/Cas9-based heritable targeted mutagenesis in Thermobia domestica: A genetic tool in an apterygote development model of wing evolution |
著者: | Ohde, Takahiro https://orcid.org/0000-0002-0870-9175 (unconfirmed) Takehana, Yusuke Shiotsuki, Takahiro Niimi, Teruyuki |
著者名の別形: | 大出, 高弘 |
キーワード: | CRISPR/Cas9 Genome editing Apterygote insect Thermobia domestica ABC transporter |
発行日: | Jul-2018 |
出版者: | Elsevier BV |
誌名: | Arthropod Structure & Development |
巻: | 47 |
号: | 4 |
開始ページ: | 362 |
終了ページ: | 369 |
抄録: | Despite previous developmental studies on basally branching wingless insects and crustaceans, the evolutionary origin of insect wings remains controversial. Knowledge regarding genetic regulation of tissues hypothesized to have given rise to wings would help to elucidate how ancestral development changed to allow the evolution of true wings. However, genetic tools available for basally branching wingless species are limited. The firebrat Thermobia domestica is an apterygote species, phylogenetically related to winged insects. T. domestica presents a suitable morphology to investigate the origin of wings, as it forms the tergal paranotum, from which wings are hypothesized to have originated. Here we report the first successful CRISPR/Cas9-based germline genome editing in T. domestica. We provide a technological platform to understand the development of tissues hypothesized to have given rise to wings in an insect with a pre-wing evolution body plan. |
著作権等: | © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/253545 |
DOI(出版社版): | 10.1016/j.asd.2018.06.003 |
PubMed ID: | 29908341 |
出現コレクション: | 学術雑誌掲載論文等 |
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