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タイトル: | Synthesis and maintenance of lipid droplets are essential for mouse preimplantation embryonic development |
著者: | Aizawa, Ryutaro Ibayashi, Megumi Tatsumi, Takayuki Yamamoto, Atsushi Kokubo, Toshiaki Miyasaka, Naoyuki Sato, Ken Ikeda, Shuntaro Minami, Naojiro Tsukamoto, Satoshi |
著者名の別形: | 池田, 俊太郎 南, 直治郎 |
キーワード: | Lipid droplet Delipidation Oocyte Embryo Mouse |
発行日: | 15-Nov-2019 |
出版者: | The Company of Biologists |
誌名: | Development |
巻: | 146 |
号: | 22 |
論文番号: | dev181925 |
抄録: | Lipid droplets (LDs), which are ubiquitous organelles consisting of a neutral lipid core coated with a phospholipid monolayer, play key roles in the regulation of cellular lipid metabolism. Although it is well known that mammalian oocytes and embryos contain LDs and that the amount of LDs varies among animal species, their physiological functions remain unclear. In this study, we have developed a method based on two-step centrifugation for efficient removal of almost all LDs from mouse MII oocytes (delipidation). We found that delipidated MII oocytes could be fertilized in vitro, and developed normally to the blastocyst stage even when the embryos were cultured in the absence of a fatty acid supply. LDs were newly synthesized and accumulated soon after delipidation, but chemical inhibition of long chain acyl-CoA synthetases (ACSLs) blocked this process, resulting in severe impairment of early embryonic development. Furthermore, we found that overabundance of LDs is detrimental to early embryonic development. Our findings demonstrate the importance of synthesis and maintenance of LDs, mediated in part by ACSL activity, during preimplantation embryonic development. |
著作権等: | © 2019. Published by The Company of Biologists Ltd 許諾条件に基づいて掲載しています。 The full-text file will be made open to the public on 25 November 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. |
URI: | http://hdl.handle.net/2433/253693 |
DOI(出版社版): | 10.1242/dev.181925 |
PubMed ID: | 31772031 |
出現コレクション: | 学術雑誌掲載論文等 |
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