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Title: Detection of: N 6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease
Authors: Imanishi, Miki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-4172-0966 (unconfirmed)
Tsuji, Shogo
Suda, Akiyo
Futaki, Shiroh
Author's alias: 今西, 未来
二木, 史朗
Issue Date: 14-Dec-2017
Publisher: Royal Society of Chemistry
Journal title: Chemical Communications
Volume: 53
Issue: 96
Start page: 12930
End page: 12933
Abstract: We found that Escherichia coli MazF toxin, an ACA-sequence-specific endoribonuclease, was sensitive to N⁶-methyladenosine (m6A), representing the first m6A-sensitive RNA cleavage enzyme. The methyl-sensitivity of MazF allowed simple analyses of both m6A demethylase and methyltransferase activity. Furthermore, the approach could be used for inhibitor screening.
Rights: This is the accepted manuscript of the article, which has been published in final form at https://doi.org/10.1039/C7CC07699A.
This is not the published version. Please cite only the published version.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/254701
DOI(Published Version): 10.1039/c7cc07699a
Appears in Collections:Journal Articles

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