|Title:||Detection of: N 6-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease|
|Authors:||Imanishi, Miki https://orcid.org/0000-0002-4172-0966 (unconfirmed)|
|Author's alias:||今西, 未来|
|Publisher:||Royal Society of Chemistry|
|Journal title:||Chemical Communications|
|Abstract:||We found that Escherichia coli MazF toxin, an ACA-sequence-specific endoribonuclease, was sensitive to N⁶-methyladenosine (m6A), representing the first m6A-sensitive RNA cleavage enzyme. The methyl-sensitivity of MazF allowed simple analyses of both m6A demethylase and methyltransferase activity. Furthermore, the approach could be used for inhibitor screening.|
|Rights:||This is the accepted manuscript of the article, which has been published in final form at https://doi.org/10.1039/C7CC07699A.|
This is not the published version. Please cite only the published version.
|Appears in Collections:||Journal Articles|
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