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| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s41598-020-63602-7.pdf | 2.15 MB | Adobe PDF | 見る/開く |
| タイトル: | Establishment of macaque trophoblast stem cell lines derived from cynomolgus monkey blastocysts |
| 著者: | Matsumoto, Shoma Porter, Christopher J. Ogasawara, Naomi Iwatani, Chizuru Tsuchiya, Hideaki Seita, Yasunari Chang, Yu-Wei Okamoto, Ikuhiro    https://orcid.org/0000-0002-2870-8321 (unconfirmed)Saitou, Mitinori Ema, Masatsugu Perkins, Theodore J. Stanford, William L. Tanaka, Satoshi |
| 著者名の別形: | 松本, 翔馬 岩谷, 千鶴 土屋, 英明 清田, 弥寿成 岡本, 郁弘 斎藤, 通紀 依馬, 正次 田中, 智 |
| キーワード: | Reproductive biology Stem cells |
| 発行日: | 22-Apr-2020 |
| 出版者: | Springer Nature |
| 誌名: | Scientific Reports |
| 巻: | 10 |
| 論文番号: | 6827 |
| 抄録: | The placenta forms a maternal-fetal junction that supports many physiological functions such as the supply of nutrition and exchange of gases and wastes. Establishing an in vitro culture model of human and non-human primate trophoblast stem/progenitor cells is important for investigating the process of early placental development and trophoblast differentiation. In this study, we have established five trophoblast stem cell (TSC) lines from cynomolgus monkey blastocysts, named macTSC #1-5. Fibroblast growth factor 4 (FGF4) enhanced proliferation of macTSCs, while other exogenous factors were not required to maintain their undifferentiated state. macTSCs showed a trophoblastic gene expression profile and trophoblast-like DNA methylation status and also exhibited differentiation capacity towards invasive trophoblast cells and multinucleated syncytia. In a xenogeneic chimera assay, these stem cells contributed to trophectoderm (TE) development in the chimeric blastocysts. macTSC are the first primate trophoblast cell lines whose proliferation is promoted by FGF4. These cell lines provide a valuable in vitro culture model to analyze the similarities and differences in placental development between human and non-human primates. |
| 著作権等: | © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| URI: | http://hdl.handle.net/2433/255223 |
| DOI(出版社版): | 10.1038/s41598-020-63602-7 |
| PubMed ID: | 32321940 |
| 出現コレクション: | 学術雑誌掲載論文等 |
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