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Title: Diffusion-limited electrochemical D-fructose sensor based on direct electron transfer-type bioelectrocatalysis by a variant of D-fructose dehydrogenase at a porous gold microelectrode
Authors: Suzuki, Yohei
Kano, Kenji
Shirai, Osamu
Kitazumi, Yuki
Author's alias: 鈴木, 洋平
加納, 健司
白井, 理
北隅, 優希
Keywords: d-fructose dehydrogenase
Amperometric biosensor
Direct electron transfer
Porous gold
Issue Date: 15-Nov-2020
Publisher: Elsevier B.V.
Journal title: Journal of Electroanalytical Chemistry
Volume: 877
Thesis number: 114651
Abstract: An electrochemical d-fructose sensor was fabricated by immobilizing a variant of d-fructose dehydrogenase on a porous gold microelectrode. The enzyme-modified electrode bioelectrocatalytically oxidizes d-fructose via direct electron transfer. The catalytic current reaches a steady-state limiting value at 0.05 V vs. Ag|AgCl (sat. KCl). The temperature dependence of the sensor suggests that its response is limited by the diffusion of d-fructose. Therefore, the sensor requires no calibration. The sensitivity of the sensor, (2.0 ± 0.2) × 10² μA cm⁻² mM⁻¹, is reproducible. The upper limit of detection is ~2.0 mM and the kinetics of the bioelectrocatalytic reaction is determined the limitation. The fabricated sensors were applied in the determination of d-fructose in commercial beverages as well as honey, and compared favorably with a more commonly used photometric method.
Rights: © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
The full-text file will be made open to the public on 15 November 2022 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1016/j.jelechem.2020.114651
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