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Title: Mechanism of delayed seed germination caused by high temperature during grain filling in rice (Oryza sativa L.)
Authors: Suriyasak, Chetphilin
Oyama, Yui
Ishida, Toshiaki
Mashiguchi, Kiyoshi
Yamaguchi, Shinjiro  kyouindb  KAKEN_id  orcid (unconfirmed)
Hamaoka, Norimitsu
Iwaya-Inoue, Mari
Ishibashi, Yushi
Author's alias: 小山, 唯
石田, 俊晃
増口, 潔
山口, 信次郎
濱岡, 範光
井上, 眞理
石橋, 勇志
Keywords: Heat
Plant physiology
Issue Date: 15-Oct-2020
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 10
Thesis number: 17378
Abstract: High temperature during grain filling considerably reduces yield and quality in rice (Oryza sativa L.); however, how high temperature affects seed germination of the next generation is not yet well understood. Here, we report that seeds from plants exposed to high temperature during the grain filling stage germinated significantly later than seeds from unstressed plants. This delay remained even after dormancy release treatments, suggesting that it was not due to primary seed dormancy determined during grain filling. In imbibed embryos of heat-stressed seeds, expression of abscisic acid (ABA) biosynthesis genes (OsNCEDs) was higher than in those of control seeds, whereas that of ABA catabolism genes (OsABA8′OHs) was lower. In the aleurone layer, despite no change in GA signaling as evidenced by no effect of heat stress on OsGAMYB gene expression, the transcripts of α-amylase genes OsAmy1C, OsAmy3B, and OsAmy3E were significantly down-regulated in heat-stressed seeds in comparison with controls. Changes in promoter methylation levels were consistent with transcriptional changes of ABA catabolism-related and α-amylase genes. These data suggest that high temperature during grain filling results in DNA methylation of ABA catabolism-related and α-amylase gene promoters, delaying germination of heat-stressed seeds.
Rights: © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit
DOI(Published Version): 10.1038/s41598-020-74281-9
PubMed ID: 33060675
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