|Title:||Organelle membrane-specific chemical labeling and dynamic imaging in living cells|
|Authors:||Tamura, Tomonori |
Tsuchiya, Masaki https://orcid.org/0000-0001-8191-3183 (unconfirmed)
|Author's alias:||田村, 朋則|
|Journal title:||Nature Chemical Biology|
|Abstract:||Lipids play crucial roles as structural elements, signaling molecules and material transporters in cells. However, the functions and dynamics of lipids within cells remain unclear because of a lack of methods to selectively label lipids in specific organelles and trace their movement by live-cell imaging. We describe here a technology for the selective labeling and fluorescence imaging (microscopic or nanoscopic) of phosphatidylcholine in target organelles. This approach involves the metabolic incorporation of azido-choline, followed by a spatially limited bioorthogonal reaction that enables the visualization and quantitative analysis of interorganelle lipid transport in live cells. More importantly, with live-cell imaging, we obtained direct evidence that the autophagosomal membrane originates from the endoplasmic reticulum. This method is simple and robust and is thus powerful for real-time tracing of interorganelle lipid trafficking.|
|Description:||狙った細胞内小器官脂質の可視化に成功 --オートファゴソーム形成機構解明に貢献--. 京都大学プレスリリース. 2020-09-28.|
|Rights:||This is the accepted manuscript of the article, which has been published in final form at https://doi.org/10.1038/s41589-020-00651-z.|
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|Appears in Collections:||Journal Articles|
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