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DCフィールド | 値 | 言語 |
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dc.contributor.author | Yamamoto, Ryosuke | en |
dc.contributor.author | Ohnishi, Hiroe | en |
dc.contributor.author | Omori, Koichi | en |
dc.contributor.author | Yamamoto, Norio | en |
dc.contributor.alternative | 山本, 亮介 | ja |
dc.contributor.alternative | 大西, 弘恵 | ja |
dc.contributor.alternative | 大森, 孝一 | ja |
dc.contributor.alternative | 山本, 典生 | ja |
dc.date.accessioned | 2020-12-10T07:30:58Z | - |
dc.date.available | 2020-12-10T07:30:58Z | - |
dc.date.issued | 2021-01-01 | - |
dc.identifier.issn | 0012-1606 | - |
dc.identifier.issn | 1095-564X | - |
dc.identifier.uri | http://hdl.handle.net/2433/259705 | - |
dc.description.abstract | The inner ear comprises four epithelial domains: the cochlea, vestibule, semicircular canals, and endolymphatic duct/sac. These structures are segregated at embryonic day 13.5 (E13.5). However, these four anatomical structures remain undefined at E10.5. Here, we aimed to identify lineage-specific genes in the early developing inner ear using published data obtained from single-cell RNA-sequencing (scRNA-seq) of embryonic mice. We downloaded 5000 single-cell transcriptome data, named ‘auditory epithelial trajectory’, from the Mouse Organogenesis Cell Atlas. The dataset was supposed to include otic epithelial cells at E9.5–13.5. We projected the 5000 cells onto a two-dimensional space encoding the transcriptional state and visualised the pattern of otic epithelial cell differentiation. We identified 15 clusters, which were annotated as one of the four components of the inner ear epithelium using known genes that characterise the four different tissues. Additionally, we classified 15 clusters into sub-regions of the four inner ear components. By comparing transcriptomes between these 15 clusters, we identified several candidates of lineage-specific genes. Characterising these new candidate genes will help future studies about inner ear development. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | en |
dc.rights | The full-text file will be made open to the public on 1 January 2022 in accordance with publisher's 'Terms and Conditions for Self-Archiving' | en |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.subject | Embryonic development | en |
dc.subject | Inner ear | en |
dc.subject | Otic vesicle | en |
dc.subject | RNA-Sequencing | en |
dc.subject | Single-cell analysis | en |
dc.title | In silico analysis of inner ear development using public whole embryonic body single-cell RNA-sequencing data | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00627848 | - |
dc.identifier.jtitle | Developmental Biology | en |
dc.identifier.volume | 469 | - |
dc.identifier.spage | 160 | - |
dc.identifier.epage | 171 | - |
dc.relation.doi | 10.1016/j.ydbio.2020.10.009 | - |
dc.textversion | author | - |
dc.address | Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University | en |
dc.address | Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University | en |
dc.address | Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University | en |
dc.address | Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Kyoto University | en |
dc.identifier.pmid | 33131705 | - |
dcterms.accessRights | open access | - |
datacite.date.available | 2022-01-01 | - |
datacite.awardNumber | 19H03803 | - |
dc.identifier.pissn | 0012-1606 | - |
dc.identifier.eissn | 1095-564X | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName.alternative | Japan Society for the Promotion of Science (JSPS) | en |
出現コレクション: | 学術雑誌掲載論文等 |
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