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タイトル: | Engineering a short-chain dehydrogenase/reductase for the stereoselective production of (2S, 3R, 4S)-4-hydroxyisoleucine with three asymmetric centers |
著者: | Shi, Xuan Miyakawa, Takuya https://orcid.org/0000-0001-5957-3549 (unconfirmed) Nakamura, Akira Hou, Feng Hibi, Makoto Ogawa, Jun https://orcid.org/0000-0003-2741-621X (unconfirmed) Kwon, Yeondae Tanokura, Masaru |
著者名の別形: | 日比, 慎 小川, 順 |
発行日: | 2017 |
出版者: | Springer Nature |
誌名: | Scientific Reports |
巻: | 7 |
論文番号: | 13703 |
抄録: | Fenugreek is a dietary supplement for anti-aging and human health. (2S, 3R, 4S)-4-hydroxyisoleucine (4-HIL), which is extracted from fenugreek seeds, is expected to be a promising orally active drug for diabetes and diabetic nephropathy because of its insulinotropic effect. Although several chemical synthesis methods of 4-HIL have been proposed, these methods require multistep reactions to control the stereochemistry of 4-HIL. In this study, we modified the key enzyme 4-HIL dehydrogenase (HILDH) to overcome the biggest limitation in commercial-scale production of 4-HIL. As a result, an effective one-step carbonyl reduction to produce (2S, 3R, 4S)-4-HIL was successfully accomplished with strict stereoselectivity (>99% de). Mass production of (2S, 3R, 4S)-4-HIL by our synthetic method could have a significant contribution to the prevention of diabetes, dyslipidemia, and Alzheimer’s disease. (120 words/200 words) |
著作権等: | © The Author(s) 2017 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
URI: | http://hdl.handle.net/2433/259778 |
DOI(出版社版): | 10.1038/s41598-017-13978-w |
PubMed ID: | 29057974 |
出現コレクション: | 学術雑誌掲載論文等 |
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