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タイトル: | Development and evaluation of a point‐of‐care test with a combination of EZ‐Fast DNA extraction and real‐time PCR and LAMP detection: evaluation using blood samples containing the bovine leukaemia DNA |
著者: | Yamazaki, Yasuko Thongchankaew‐Seo, Uraiwan Nagao, Konomu Mekata, Hirohisa Yamazaki, Wataru https://orcid.org/0000-0002-7874-8014 (unconfirmed) |
著者名の別形: | 山崎, 安子 山崎, 渉 |
キーワード: | EZ‐Fast field setting LAMP point‐of‐care test POCT real‐time PCR |
発行日: | Dec-2020 |
出版者: | Wiley |
誌名: | Letters in Applied Microbiology |
巻: | 71 |
号: | 6 |
開始ページ: | 500 |
終了ページ: | 566 |
抄録: | Along with progress in globalization of society, the spread of infectious diseases has accelerated worldwide. The deployment of highly sensitive genetic tests is essential for early diagnosis and early containment of potential outbreaks and epidemics, as well as routine surveillance, although tedious and expensive nucleic acid extraction steps represent a major drawback. Here we developed a simple and rapid DNA extraction method, named as an EZ‐Fast kit, applicable to the field setting. The kit does not require advanced laboratory equipment or expensive DNA extraction kits and achieves crude DNA extraction within 10 min at extremely low cost and can easily be performed in field settings. When combined with real‐time PCR and LAMP analyses, the performance of the POCT, using 183 bovine blood samples, was similar to that of the existing DNA extraction method: 92·5% (135/146) (real‐time PCR) and 93·7% (133/142) (LAMP) diagnostic sensitivities, and 100% diagnostic specificities. The developed POCT provides a powerful tool to facilitate on‐site diagnosis in a field setting. |
著作権等: | This is the peer reviewed version of the following article: ['Letters in Applied Microbiology' 71(6), 560-566], which has been published in final form at https://doi.org/10.1111/lam.13376. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. The full-text file will be made open to the public on 27 August 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving' This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/259792 |
DOI(出版社版): | 10.1111/lam.13376 |
PubMed ID: | 32852051 |
出現コレクション: | 学術雑誌掲載論文等 |
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