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Title: Redox-Sensitive Cysteines Confer Proximal Control of the Molecular Crowding Barrier in the Nuclear Pore
Authors: Zhang, Wanzhen
Watanabe, Ryuji
Konishi, Hide A.
Fujiwara, Takahiro  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-5576-759X (unconfirmed)
Yoshimura, Shige H.
Kumeta, Masahiro  kyouindb  KAKEN_id
Author's alias: 張, 菀禎
渡邉, 隆治
小西, 秀明
藤原, 敬宏
吉村, 成弘
粂田, 昌宏
Keywords: molecular crowding
nuclear pore complex
nuclear transport
nucleoporin
oxidative stress
redox response
Issue Date: 15-Dec-2020
Publisher: Elsevier BV
Journal title: Cell Reports
Volume: 33
Issue: 11
Thesis number: 108484
Abstract: The nuclear pore complex forms a highly crowded selective barrier with intrinsically disordered regions at the nuclear membrane to coordinate nucleocytoplasmic molecular communications. Although oxidative stress is known to alter the barrier function, the molecular mechanism underlying this adaptive control of the nuclear pore complex remains unknown. Here we uncover a systematic control of the crowding barrier within the nuclear pore in response to various redox environments. Direct measurements of the crowding states using a crowding-sensitive FRET (Förster resonance energy transfer) probe reveal specific roles of the nuclear pore subunits that adjust the degree of crowding in response to different redox conditions, by adaptively forming or disrupting redox-sensitive disulfide bonds. Relationships between crowding control and the barrier function of the nuclear pore are investigated by single-molecular fluorescence measurements of nuclear transport. Based on these findings, we propose a proximal control model of molecular crowding in vivo that is dynamically regulated at the molecular level.
Description: 酸化ストレスが細胞の核膜機能を変える機構を解明 --環境に応じて分子の「混み具合」が変わる仕組み--. 京都大学プレスリリース. 2020-12-16.
Rights: © 2020 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
URI: http://hdl.handle.net/2433/259818
DOI(Published Version): 10.1016/j.celrep.2020.108484
Related Link: https://www.kyoto-u.ac.jp/ja/research-news/2020-12-16-0
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