ダウンロード数: 59
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
jcs.245282.pdf | 15.87 MB | Adobe PDF | 見る/開く |
タイトル: | Sequential peripheral enrichment of H2A.Zac and H3K9me2 during trophoblast differentiation in human embryonic stem cells |
著者: | Kafer, Georgia Rose Tanaka, Yoshihisa Rillo-Bohn, Regina Shimizu, Eiko Hasegawa, Kouichi Carlton, Peter M. |
著者名の別形: | 田中, 良尚 長谷川, 光一 |
キーワード: | Chromatin Differentiation Human embryonic stemcells Quantitative microscopy Trophoblast |
発行日: | 16-Nov-2020 |
出版者: | The Company of Biologists |
誌名: | Journal of Cell Science |
巻: | 133 |
号: | 24 |
論文番号: | jcs245282 |
抄録: | During the transition from pluripotency to a lineage-committed state, chromatin undergoes large-scale changes in structure, involving covalent modification of histone tails, use of histone variants and gene position changes with respect to the nuclear periphery. Here, using high-resolution microscopy and quantitative image analysis, we surveyed a panel of histone modifications for changes in nuclear peripheral enrichment during differentiation of human embryonic stem cells to a trophoblast-like lineage. We found two dynamic modifications at the nuclear periphery, acetylation of histone H2A.Z (H2A.Zac), and dimethylation of histone H3 at lysine 9 (H3K9me2). We demonstrate successive peripheral enrichment of these markers, with H2A.Zac followed by H3K9me2, over the course of 4 days. We find that H3K9me2 increases concomitantly with, but independently of, expression of lamin A, since deletion of lamin A did not affect H3K9me2 enrichment. We further show that inhibition of histone deacetylases causes persistent and increased H2A.Z acetylation at the periphery, delayed H3K9me2 enrichment and failure to differentiate. Our results show a concerted change in the nature of peripheral chromatin occurs upon differentiation into the trophoblast state. |
記述: | ES細胞分化過程において継時的に分布変化するヒストン修飾を同定 --ヒト幹細胞が分化する時、核では何が起こっているのか--. 京都大学プレスリリース. 2021-01-06. |
著作権等: | © 2020. Published by The Company of Biologists Ltd 許諾条件に基づいて掲載しています。 The full-text file will be made open to the public on 16 November 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. |
URI: | http://hdl.handle.net/2433/260843 |
DOI(出版社版): | 10.1242/jcs.245282 |
PubMed ID: | 33199519 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2021-01-06 |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。