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j.bbrc.2019.08.091.pdf | 733.23 kB | Adobe PDF | 見る/開く |
タイトル: | Talin is required to increase stiffness of focal molecular complex in its early formation process |
著者: | Nakao, Nobuhiko Maki, Koichiro https://orcid.org/0000-0002-7607-2903 (unconfirmed) Mofrad, Mohammad K. R. Adachi, Taiji https://orcid.org/0000-0001-5280-4156 (unconfirmed) |
著者名の別形: | 仲尾, 信彦 牧, 功一郎 安達, 泰治 |
キーワード: | Focal molecular complex Talin Stiffness Atomic force microscopy Mechanotransduction |
発行日: | 20-Oct-2019 |
出版者: | Elsevier BV |
誌名: | Biochemical and Biophysical Research Communications |
巻: | 518 |
号: | 3 |
開始ページ: | 579 |
終了ページ: | 583 |
抄録: | For cellular adaptation in mechanical environments, it is important to consider transmission of forces from the outside to the inside of cells via a focal molecular complex. The focal molecular complex, which consists of integrin, talin, vinculin and actin, is known to form in response to a force applied via the extra-cellular matrix (ECM). In the early formation process of the complex, the complex–actin connection is reinforced. These structural changes of the nascent complex result in an increase in its mechanical integrity and overall stiffness, possibly leading to the maturation of the nascent complex by enhancing force transmission. In this study, we hypothesized that the complex component talin is a crucial factor in increasing the stiffness of the nascent complex. To test the hypothesis, we used atomic force microscopy (AFM) to measure the stiffness of the nascent complex using a probe coated with fibronectin. Stiffness measurements were conducted for intact and talin knocked-down cells. Our results demonstrated that talin was required to increase the stiffness of the nascent complex, which could be caused by the reinforced connection between the complex and actin filaments mediated by talin. |
著作権等: | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. The full-text file will be made open to the public on 20 October 2020 in accordance with publisher's 'Terms and Conditions for Self-Archiving. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/261016 |
DOI(出版社版): | 10.1016/j.bbrc.2019.08.091 |
PubMed ID: | 31451222 |
出現コレクション: | 学術雑誌掲載論文等 |
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