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Title: Talin is required to increase stiffness of focal molecular complex in its early formation process
Authors: Nakao, Nobuhiko
Maki, Koichiro  kyouindb  KAKEN_id
Mofrad, K. R. Mohammad
Adachi, Taiji  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0001-5280-4156 (unconfirmed)
Author's alias: 仲尾, 信彦
安達, 泰治
Keywords: Focal molecular complex
Talin
Stiffness
Atomic force microscopy
Mechanotransduction
Issue Date: 20-Oct-2019
Publisher: Elsevier BV
Journal title: Biochemical and Biophysical Research Communications
Volume: 518
Issue: 3
Start page: 579
End page: 583
Abstract: For cellular adaptation in mechanical environments, it is important to consider transmission of forces from the outside to the inside of cells via a focal molecular complex. The focal molecular complex, which consists of integrin, talin, vinculin and actin, is known to form in response to a force applied via the extra-cellular matrix (ECM). In the early formation process of the complex, the complex–actin connection is reinforced. These structural changes of the nascent complex result in an increase in its mechanical integrity and overall stiffness, possibly leading to the maturation of the nascent complex by enhancing force transmission. In this study, we hypothesized that the complex component talin is a crucial factor in increasing the stiffness of the nascent complex. To test the hypothesis, we used atomic force microscopy (AFM) to measure the stiffness of the nascent complex using a probe coated with fibronectin. Stiffness measurements were conducted for intact and talin knocked-down cells. Our results demonstrated that talin was required to increase the stiffness of the nascent complex, which could be caused by the reinforced connection between the complex and actin filaments mediated by talin.
Rights: © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/261016
DOI(Published Version): 10.1016/j.bbrc.2019.08.091
PubMed ID: 31451222
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