Downloads: 76

Files in This Item:
File Description SizeFormat 
s41598-021-81515-x.pdf1.26 MBAdobe PDFView/Open
Title: Deficiency of the RIβ subunit of protein kinase A causes body tremor and impaired fear conditioning memory in rats
Authors: Hoang Trung, Hieu
Yoshihara, Toru
Nakao, Akito  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-2296-8245 (unconfirmed)
Hayashida, Katsumi
Hirata, Yoshiki
Shirasuna, Koumei
Kuwamura, Mitsuru
Nakagawa, Yuki
Kaneko, Takehito
Mori, Yasuo
Asano, Masahide  kyouindb  KAKEN_id
Kuramoto, Takashi
Author's alias: 吉原, 亨
中尾, 章人
林田, 克海
平田, 良樹
白砂, 孔明
桑村, 充
中川, 優貴
金子, 武人
森, 泰生
浅野, 雅秀
庫本, 高志
Keywords: Animal breeding
Behavioural genetics
Genetic engineering
Genetics of the nervous system
Long-term potentiation
Movement disorders
Mutation
Issue Date: 21-Jan-2021
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 11
Thesis number: 2039
Abstract: The RIβ subunit of cAMP-dependent protein kinase (PKA), encoded by Prkar1b, is a neuronal isoform of the type I regulatory subunit of PKA. Mice lacking the RIβ subunit exhibit normal long-term potentiation (LTP) in the Schaffer collateral pathway of the hippocampus and normal behavior in the open-field and fear conditioning tests. Here, we combined genetic, electrophysiological, and behavioral approaches to demonstrate that the RIβ subunit was involved in body tremor, LTP in the Schaffer collateral pathway, and fear conditioning memory in rats. Genetic analysis of WTC-furue, a mutant strain with spontaneous tremors, revealed a deletion in the Prkar1b gene of the WTC-furue genome. Prkar1b-deficient rats created by the CRISPR/Cas9 system exhibited body tremor. Hippocampal slices from mutant rats showed deficient LTP in the Schaffer collateral–CA1 synapse. Mutant rats also exhibited decreased freezing time following contextual and cued fear conditioning, as well as increased exploratory behavior in the open field. These findings indicate the roles of the RIβ subunit in tremor pathogenesis and contextual and cued fear memory, and suggest that the hippocampal and amygdala roles of this subunit differ between mice and rats and that rats are therefore beneficial for exploring RIβ function.
Rights: © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/261172
DOI(Published Version): 10.1038/s41598-021-81515-x
PubMed ID: 33479380
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks


Export Format: 


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.