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Title: Elucidation of the mechanism of subunit exchange in αB crystallin oligomers
Authors: Inoue, Rintaro  kyouindb  KAKEN_id
Sakamaki, Yusuke
Takata, Takumi  kyouindb  KAKEN_id
Wood, Kathleen
Morishima, Ken  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-1091-3850 (unconfirmed)
Sato, Nobuhiro  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-6960-6567 (unconfirmed)
Okuda, Aya  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-6222-8364 (unconfirmed)
Shimizu, Masahiro
Urade, Reiko  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-9232-6176 (unconfirmed)
Fujii, Noriko
Sugiyama, Masaaki
Author's alias: 井上, 倫太郎
酒巻, 裕介
髙田, 匠
守島, 健
佐藤, 信浩
奥田, 綾
清水, 将裕
裏出, 令子
藤井, 紀子
杉山, 正明
Keywords: Biological techniques
Biophysics
Issue Date: 28-Jan-2021
Publisher: Springer Nature
Journal title: Scientific Reports
Volume: 11
Thesis number: 2555
Abstract: AlphaB crystallin (αB-crystallin) is a key protein for maintaining the long-term transparency of the eye lens. In the eye lens, αB-crystallin is a “dynamical” oligomer regulated by subunit exchange between the oligomers. To elucidate the unsettled mechanism of subunit exchange in αB-crystallin oligomers, the study was carried out at two different protein concentrations, 28.5 mg/mL (dense sample) and 0.45 mg/mL (dilute sample), through inverse contrast matching small-angle neutron scattering. Interestingly, the exchange rate of the dense sample was the same as that of the dilute sample. From analytical ultracentrifuge measurements, the coexistence of small molecular weight components and oligomers was detected, regardless of the protein concentration. The model proposed that subunit exchange could proceed through the assistance of monomers and other small oligomers; the key mechanism is attaching/detaching monomers and other small oligomers to/from oligomers. Moreover, this model successfully reproduced the experimental results for both dense and dilute solutions. It is concluded that the monomer and other small oligomers attaching/detaching mainly regulates the subunit exchange in αB-crystallin oligomer.
Rights: © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
URI: http://hdl.handle.net/2433/261677
DOI(Published Version): 10.1038/s41598-021-82250-z
PubMed ID: 33510404
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