Downloads: 28

Files in This Item:
File Description SizeFormat 
s42003-021-01707-z.pdf3.31 MBAdobe PDFView/Open
Title: Histone variant H2A.B-H2B dimers are spontaneously exchanged with canonical H2A-H2B in the nucleosome
Authors: Hirano, Rina
Arimura, Yasuhiro
Kujirai, Tomoya
Shibata, Mikihiro
Okuda, Aya  kyouindb  KAKEN_id  orcid (unconfirmed)
Morishima, Ken  kyouindb  KAKEN_id  orcid (unconfirmed)
Inoue, Rintaro  kyouindb  KAKEN_id
Sugiyama, Masaaki
Kurumizaka, Hitoshi
Author's alias: 平野, 里奈
有村, 泰宏
鯨井, 智也
柴田, 幹大
奥田, 綾
守島, 健
井上, 倫太郎
杉山, 正明
胡桃坂, 仁志
Keywords: Biochemistry
Chromatin structure
Histone variants
Issue Date: 12-Feb-2021
Publisher: Springer Nature
Journal title: Communications Biology
Volume: 4
Thesis number: 191
Abstract: H2A.B is an evolutionarily distant histone H2A variant that accumulates on DNA repair sites, DNA replication sites, and actively transcribing regions in genomes. In cells, H2A.B exchanges rapidly in chromatin, but the mechanism has remained enigmatic. In the present study, we found that the H2A.B-H2B dimer incorporated within the nucleosome exchanges with the canonical H2A-H2B dimer without assistance from additional factors, such as histone chaperones and nucleosome remodelers. High-speed atomic force microscopy revealed that the H2A.B nucleosome, but not the canonical H2A nucleosome, transiently forms an intermediate “open conformation”, in which two H2A.B-H2B dimers may be detached from the H3-H4 tetramer and bind to the DNA regions near the entry/exit sites. Mutational analyses revealed that the H2A.B C-terminal region is responsible for the adoption of the open conformation and the H2A.B-H2B exchange in the nucleosome. These findings provide mechanistic insights into the histone exchange of the H2A.B nucleosome.
Description: 精子形成に重要なヒストンによるDNAの新たな折りたたみを解明. 京都大学プレスリリース. 2021-02-22.
Rights: © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
DOI(Published Version): 10.1038/s42003-021-01707-z
Related Link:
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks

Export Format: 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.