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タイトル: | Virus-Mediated Transient Expression Techniques Enable Functional Genomics Studies and Modulations of Betalain Biosynthesis and Plant Height in Quinoa |
著者: | Ogata, Takuya Toyoshima, Masami Yamamizo-Oda, Chihiro Kobayashi, Yasufumi Fujii, Kenichiro Tanaka, Kojiro Tanaka, Tsutomu Mizukoshi, Hiroharu Yasui, Yasuo https://orcid.org/0000-0003-3804-7886 (unconfirmed) Nagatoshi, Yukari Yoshikawa, Nobuyuki Fujita, Yasunari |
著者名の別形: | 小賀田, 拓也 豊島, 真美 小田, 千尋 小林, 安文 藤井, 健一朗 田中, 孝二郎 田中, 努 水越, 裕治 安井, 康夫 永利, 友佳理 吉川, 信幸 藤田, 泰成 |
キーワード: | apple latent spherical virus betalain functional genomics quinoa virus-induced gene silencing virus-mediated gene overexpression |
発行日: | 18-Mar-2021 |
出版者: | Frontiers Media SA |
誌名: | Frontiers in Plant Science |
巻: | 12 |
論文番号: | 643499 |
抄録: | Quinoa (Chenopodium quinoa), native to the Andean region of South America, has been recognized as a potentially important crop in terms of global food and nutrition security since it can thrive in harsh environments and has an excellent nutritional profile. Even though challenges of analyzing the complex and heterogeneous allotetraploid genome of quinoa have recently been overcome, with the whole genome-sequencing of quinoa and the creation of genotyped inbred lines, the lack of technology to analyze gene function in planta is a major limiting factor in quinoa research. Here, we demonstrate that two virus-mediated transient expression techniques, virus-induced gene silencing (VIGS) and virus-mediated overexpression (VOX), can be used in quinoa. We show that apple latent spherical virus (ALSV) can induce gene silencing of quinoa phytoene desaturase (CqPDS1) in a broad range of quinoa inbred lines derived from the northern and southern highland and lowland sub-populations. In addition, we show that ALSV can be used as a VOX vector in roots. Our data also indicate that silencing a quinoa 3, 4-dihydroxyphenylalanine 4, 5-dioxygenase gene (CqDODA1) or a cytochrome P450 enzyme gene (CqCYP76AD1) inhibits betalain production and that knockdown of a reduced-height gene homolog (CqRHT1) causes an overgrowth phenotype in quinoa. Moreover, we show that ALSV can be transmitted to the progeny of quinoa plants. Thus, our findings enable functional genomics in quinoa, ushering in a new era of quinoa research. |
記述: | スーパー作物キヌアにおける遺伝子機能の解析技術を開発 --優れた環境適応性や栄養特性の謎を解き、作物開発を加速化--. 京都大学プレスリリース. 2021-03-19. |
著作権等: | © 2021 Ogata, Toyoshima, Yamamizo-Oda, Kobayashi, Fujii, Tanaka, Tanaka, Mizukoshi, Yasui, Nagatoshi, Yoshikawa and Fujita. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
URI: | http://hdl.handle.net/2433/262143 |
DOI(出版社版): | 10.3389/fpls.2021.643499 |
PubMed ID: | 33815450 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2021-03-19-1 |
出現コレクション: | 学術雑誌掲載論文等 |
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