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Title: Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin
Authors: Oda, Kazumasa
Nomura, Takashi
Nakane, Takanori
Yamashita, Keitaro
Inoue, Keiichi
Ito, Shota
Vierock, Johannes
Hirata, Kunio
Maturana, Andrés D
Katayama, Kota
Ikuta, Tatsuya
Ishigami, Itsuki
Izume, Tamaki
Umeda, Rie
Eguma, Ryuun
Oishi, Satomi
Kasuya, Go
Kato, Takafumi
Kusakizako, Tsukasa
Shihoya, Wataru
Shimada, Hiroto
Takatsuji, Tomoyuki
Takemoto, Mizuki
Taniguchi, Reiya
Tomita, Atsuhiro
Nakamura, Ryoki
Fukuda, Masahiro
Miyauchi, Hirotake
Lee, Yongchan
Nango, Eriko
Tanaka, Rie  kyouindb  KAKEN_id  orcid (unconfirmed)
Tanaka, Tomoyuki
Sugahara, Michihiro
Kimura, Tetsunari
Shimamura, Tatsuro  kyouindb  KAKEN_id
Fujiwara, Takaaki
Yamanaka, Yasuaki
Owada, Shigeki
Joti, Yasumasa
Tono, Kensuke
Ishitani, Ryuichiro
Hayashi, Shigehiko
Kandori, Hideki
Hegemann, Peter
Iwata, So  kyouindb  KAKEN_id
Kubo, Minoru
Nishizawa, Tomohiro
Nureki, Osamu
Author's alias: 小田, 和正
野村, 高志
中根, 崇智
山下, 恵太郎
井上, 圭一
伊藤, 奨太
平田, 邦生
片山, 耕大
生田, 達也
石神, 一貴
井爪, 珠希
江熊, 龍雲
糟谷, 豪
草木迫, 司
志甫谷, 渉
島田, 寛人
高辻, 智之
富田, 篤弘
南後, 恵理子
田中, 里枝
田中, 智之
菅原, 道泰
木村, 哲就
島村, 達郎
藤原, 孝彰
山中, 保明
大和田, 成起
城地, 保昌
登野, 健介
石谷, 隆一郎
林, 重彦
神取, 秀樹
岩田, 想
久保, 稔
西澤, 知宏
濡木, 理
Issue Date: 23-Mar-2021
Publisher: eLife Sciences Publications, Ltd
Journal title: eLife
Volume: 10
Thesis number: e62389
Abstract: Channelrhodopsins (ChRs) are microbial light-gated ion channels utilized in optogenetics to control neural activity with light . Light absorption causes retinal chromophore isomerization and subsequent protein conformational changes visualized as optically distinguished intermediates, coupled with channel opening and closing. However, the detailed molecular events underlying channel gating remain unknown. We performed time-resolved serial femtosecond crystallographic analyses of ChR by using an X-ray free electron laser, which revealed conformational changes following photoactivation. The isomerized retinal adopts a twisted conformation and shifts toward the putative internal proton donor residues, consequently inducing an outward shift of TM3, as well as a local deformation in TM7. These early conformational changes in the pore-forming helices should be the triggers that lead to opening of the ion conducting pore.
Description: X線自由電子レーザーを用いて、光照射によるチャネルロドプシンの構造変化の過程を捉えることに成功. 京都大学プレスリリース. 2021-03-26.
Rights: © 2021, Oda et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
DOI(Published Version): 10.7554/eLife.62389
PubMed ID: 33752801
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