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タイトル: The use of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide–based colorimetric assay in the viability analysis of the filamentous cyanobacterium Arthrospira platensis
著者: Shiraishi, Hideaki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-3490-9269 (unconfirmed)
Toyoda, Akiko
著者名の別形: 白石, 英秋
キーワード: colorimetric viability assay
cyanobacteria
heat sensitivity
spirulina
発行日: Mar-2021
出版者: Oxford University Press (OUP)
誌名: Bioscience, Biotechnology, and Biochemistry
巻: 85
号: 3
開始ページ: 739
終了ページ: 742
抄録: The applicability of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay to an industrially valuable filamentous cyanobacterium Arthrospira platensis was examined. When it was applied to A. platensis NIES-39, as few as 10 viable trichomes were quantitatively detected. However, depending on the experimental conditions, it also generated artifactual viability signals. The results should help clarify the scope and limits of the MTT assay in viability analysis.
著作権等: This is a pre-copyedited, author-produced version of an article accepted for publication in 'Bioscience, Biotechnology, and Biochemistry'following peer review. The version of record [Bioscience, Biotechnology, and Biochemistry, Volume 85, Issue 3, March 2021, Pages 739–742] is available online at: https://doi.org/10.1093/bbb/zbaa050.
The full-text file will be made open to the public on 31 December 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving'
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/262924
DOI(出版社版): 10.1093/bbb/zbaa050
PubMed ID: 33624771
出現コレクション:学術雑誌掲載論文等

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