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Title: The use of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide–based colorimetric assay in the viability analysis of the filamentous cyanobacterium Arthrospira platensis
Authors: Shiraishi, Hideaki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-3490-9269 (unconfirmed)
Toyoda, Akiko
Author's alias: 白石, 英秋
Keywords: colorimetric viability assay
cyanobacteria
heat sensitivity
spirulina
Issue Date: 31-Dec-2020
Publisher: Oxford University Press (OUP)
Journal title: Bioscience, Biotechnology, and Biochemistry
Volume: 85
Issue: 3
Start page: 739
End page: 742
Abstract: The applicability of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay to an industrially valuable filamentous cyanobacterium Arthrospira platensis was examined. When it was applied to A. platensis NIES-39, as few as 10 viable trichomes were quantitatively detected. However, depending on the experimental conditions, it also generated artifactual viability signals. The results should help clarify the scope and limits of the MTT assay in viability analysis.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in 'Bioscience, Biotechnology, and Biochemistry'following peer review. The version of record [Bioscience, Biotechnology, and Biochemistry, Volume 85, Issue 3, March 2021, Pages 739–742] is available online at: https://doi.org/10.1093/bbb/zbaa050.
The full-text file will be made open to the public on 31 December 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving'
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/262924
DOI(Published Version): 10.1093/bbb/zbaa050
PubMed ID: 33624771
Appears in Collections:Journal Articles

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