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eLife.55108.pdf | 4.62 MB | Adobe PDF | 見る/開く |
タイトル: | Quantitative analyses reveal extracellular dynamics of Wnt ligands in Xenopus embryos |
著者: | Mii, Yusuke Nakazato, Kenichi Pack, Chan-Gi Ikeda, Takafumi Sako, Yasushi Mochizuki, Atsushi Taira, Masanori Takada, Shinji |
著者名の別形: | 三井, 優輔 中里, 研一 池田, 貴史 佐甲, 靖志 望月, 敦史 平良, 眞規 高田, 慎治 |
キーワード: | Developmental Biology Physics of Living Systems Wnt morphogen diffusion FCS FRAP FDAP Xenopus |
発行日: | 2021 |
出版者: | eLife Sciences Publications, Ltd |
誌名: | eLife |
巻: | 10 |
論文番号: | e55108 |
抄録: | The mechanism of intercellular transport of Wnt ligands is still a matter of debate. To better understand this issue, we examined the distribution and dynamics of Wnt8 in Xenopus embryos. While Venus-tagged Wnt8 was found on the surfaces of cells close to Wnt-producing cells, we also detected its dispersal over distances of 15 cell diameters. A combination of fluorescence correlation spectroscopy and quantitative imaging suggested that only a small proportion of Wnt8 ligands diffuses freely, whereas most Wnt8 molecules are bound to cell surfaces. Fluorescence decay after photoconversion showed that Wnt8 ligands bound on cell surfaces decrease exponentially, suggesting a dynamic exchange of bound forms of Wnt ligands. Mathematical modeling based on this exchange recapitulates a graded distribution of bound, but not free, Wnt ligands. Based on these results, we propose that Wnt distribution in tissues is controlled by a dynamic exchange of its abundant bound and rare free populations. |
記述: | 動く分子と動かない分子が協調して、安定した位置情報を素早く作り出す. 京都大学プレスリリース. 2021-06-04. |
著作権等: | © 2021, Mii et al. This article is distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use and redistribution provided that the original author and source are credited. |
URI: | http://hdl.handle.net/2433/263212 |
DOI(出版社版): | 10.7554/eLife.55108 |
PubMed ID: | 33904408 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2021-06-04 |
出現コレクション: | 学術雑誌掲載論文等 |
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