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Title: Effects of in vivo cyclic compressive loading on the distribution of local Col2 and superficial lubricin in rat knee cartilage
Authors: Ji, Xiang
Ito, Akira  kyouindb  KAKEN_id  orcid (unconfirmed)
Nakahata, Akihiro
Nishitani, Kohei  kyouindb  KAKEN_id
Kuroki, Hiroshi
Aoyama, Tomoki  kyouindb  KAKEN_id
Author's alias: 伊藤, 明良
中畑, 晶博
西谷, 江平
黒木, 裕士
青山, 朋樹
Keywords: cartilage degeneration
in vivo cyclic compression
post-traumatic osteoarthritis
rat model
superficial lubricin
type II collagen
Issue Date: Mar-2021
Publisher: Wiley
Journal title: Journal of Orthopaedic Research
Volume: 39
Issue: 3
Start page: 543
End page: 552
Abstract: This study aimed to examine the effects of an episode of in vivo cyclic loading on rat knee articular cartilage (AC) under medium-term observation, while also investigating relevant factors associated with the progression of post-traumatic osteoarthritis (PTOA). Twelve-week-old Wistar rats underwent one episode comprising 60 cycles of 20 N or 50 N dynamic compression on the right knee joint. Spatiotemporal changes in the AC after loading were evaluated using histology and immunohistochemistry at 3 days and 1, 2, 4, and 8 weeks after loading (n = 6 for each condition). Chondrocyte vitality was assessed at 1, 3, 6, and 12 hours after loading (n = 2 for each condition). A localized AC lesion on the lateral femoral condyle was confirmed in all subjects. The surface and intermediate cartilage in the affected area degenerated after loading, but the calcified cartilage remained intact. Expression of type II collagen in the lesion cartilage was upregulated after loading, whereas the superficial lubricin layer was eroded in response to cyclic compression. However, the distribution of superficial lubricin gradually recovered to the normal level 4 weeks after loading-induced injury. We confirmed that 60 repetitions of cyclic loading exceeding 20 N could result in cartilage damage in the rat knee. Endogenous repairs in well-structured joints work well to rebuild protective layers on the lesion cartilage surface, which may be the latent factor delaying the progression of PTOA.
Rights: This is the peer reviewed version of the following article: [Journal of Orthopaedic Research, 39(3), 543-552, March 2021], which has been published in final form at This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
The full-text file will be made open to the public on 04 August 2021 in accordance with publisher's 'Terms and Conditions for Self-Archiving'.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
DOI(Published Version): 10.1002/jor.24812
PubMed ID: 32716572
Appears in Collections:Journal Articles

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