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タイトル: | Two distinct motifs for Zic-r.a drive specific gene expression in two cell lineages |
著者: | Oda-Ishii, Izumi Yu, Deli Satou, Yutaka https://orcid.org/0000-0001-5193-0708 (unconfirmed) |
著者名の別形: | 小田, いずみ 佐藤, ゆたか |
キーワード: | Ascidians Transcription factor Non-canonical-motif sites Zic Tbx6 |
発行日: | Jun-2021 |
出版者: | The Company of Biologists |
誌名: | Development |
巻: | 148 |
号: | 11 |
論文番号: | 199538 |
抄録: | Zic-r.a, a maternal transcription factor, specifies posterior fate in ascidian embryos. However, its direct target, Tbx6-r.b, does not contain typical Zic-r.a-binding sites in its regulatory region. Using an in vitro selection assay, we found that Zic-r.a binds to sites dissimilar to the canonical motif, by which it activates Tbx6-r.b in a sub-lineage of muscle cells. These sites with non-canonical motifs have weak affinity for Zic-r.a; therefore, it activates Tbx6-r.b only in cells expressing Zic-r.a abundantly. Meanwhile, we found that Zic-r.a expressed zygotically in late embryos activates neural genes through canonical sites. Because different zinc-finger domains of Zic-r.a are important for driving reporters with canonical and non-canonical sites, it is likely that the non-canonical motif is not a divergent version of the canonical motif. In other words, our data indicate that the non-canonical motif represents a motif distinct from the canonical motif. Thus, Zic-r.a recognizes two distinct motifs to activate two sets of genes at two timepoints in development. |
著作権等: | © 2021. Published by The Company of Biologists Ltd. The full-text file will be made open to the public on 07 June 2022 in accordance with publisher's 'Terms and Conditions for Self-Archiving' |
URI: | http://hdl.handle.net/2433/263897 |
DOI(出版社版): | 10.1242/dev.199538 |
PubMed ID: | 34100063 |
出現コレクション: | 学術雑誌掲載論文等 |
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