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タイトル: | Iodine containing porous organosilica nanoparticles trigger tumor spheroids destruction upon monochromatic X-ray irradiation: DNA breaks and K-edge energy X-ray |
著者: | Higashi, Yuya Matsumoto, Kotaro ![]() ![]() Saitoh, Hiroyuki Shiro, Ayumi Ma, Yue Laird, Mathilde Chinnathambi, Shanmugavel ![]() ![]() ![]() Birault, Albane Doan, Tan Le Hoang Yasuda, Ryo Tajima, Toshiki Kawachi, Tetsuya Tamanoi, Fuyuhiko ![]() ![]() ![]() |
著者名の別形: | 東, 佑弥 松本, 光太郎 齋藤, 寛之 城, 鮎美 馬, 越 安田, 良 田島, 俊樹 河内, 哲哉 玉野井, 冬彦 |
キーワード: | Cancer Nanoscience and technology |
発行日: | 2021 |
出版者: | Springer Nature |
誌名: | Scientific Reports |
巻: | 11 |
論文番号: | 14192 |
抄録: | X-ray irradiation of high Z elements causes photoelectric effects that include the release of Auger electrons that can induce localized DNA breaks. We have previously established a tumor spheroid-based assay that used gadolinium containing mesoporous silica nanoparticles and synchrotron-generated monochromatic X-rays. In this work, we focused on iodine and synthesized iodine-containing porous organosilica (IPO) nanoparticles. IPO were loaded onto tumor spheroids and the spheroids were irradiated with 33.2 keV monochromatic X-ray. After incubation in CO₂ incubator, destruction of tumor spheroids was observed which was accompanied by apoptosis induction, as determined by the TUNEL assay. By employing the γH2AX assay, we detected double strand DNA cleavages immediately after the irradiation. These results suggest that IPO first generate double strand DNA breaks upon X-ray irradiation followed by apoptosis induction of cancer cells. Use of three different monochromatic X-rays having energy levels of 33.0, 33.2 and 33.4 keV as well as X-rays with 0.1 keV energy intervals showed that the optimum effect of all three events (spheroid destruction, apoptosis induction and generation of double strand DNA breaks) occurred with a 33.2 keV monochromatic X-ray. These results uncover the preferential effect of K-edge energy X-ray for tumor spheroid destruction mediated by iodine containing nanoparticles. |
記述: | アインシュタインの光電効果をがん細胞の中で再現 放射線治療への新展開. 京都大学プレスリリース. 2021-07-14. Quantum physics helps destroy cancer cells. 京都大学プレスリリース. 2021-07-14. |
著作権等: | © The Author(s) 2021 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/264444 |
DOI(出版社版): | 10.1038/s41598-021-93429-9 |
PubMed ID: | 34262055 |
関連リンク: | https://www.icems.kyoto-u.ac.jp/news/4271/ https://www.icems.kyoto-u.ac.jp/en/news/4273/ |
出現コレクション: | 学術雑誌掲載論文等 |
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