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タイトル: | Simple derivation of skeletal muscle from human pluripotent stem cells using temperature‐sensitive Sendai virus vector |
著者: | Tan, Ghee Wan Kondo, Takayuki Imamura, Keiko Suga, Mika Enami, Takako Nagahashi, Ayako Tsukita, Kayoko Inoue, Ikuyo Kawaguchi, Jitsutaro Shu, Tsugumine Inoue, Haruhisa https://orcid.org/0000-0003-4736-9537 (unconfirmed) |
著者名の別形: | 近藤, 孝之 今村, 恵子 菅, 三佳 江浪, 貴子 永橋, 文子 月田, 香代子 井上, 育代 川口, 実太郎 井上, 治久 |
キーワード: | differentiation method disease modelling high temperature treatment human embryonic stem cells human-induced pluripotent stem cells Myod1 Sendai virus skeletal muscle |
発行日: | Oct-2021 |
出版者: | Wiley |
誌名: | Journal of Cellular and Molecular Medicine |
巻: | 25 |
号: | 20 |
開始ページ: | 9586 |
終了ページ: | 9596 |
抄録: | Human pluripotent stem cells have the potential to differentiate into various cell types including skeletal muscles (SkM), and they are applied to regenerative medicine or in vitro modelling for intractable diseases. A simple differentiation method is required for SkM cells to accelerate neuromuscular disease studies. Here, we established a simple method to convert human pluripotent stem cells into SkM cells by using temperature-sensitive Sendai virus (SeV) vector encoding myoblast determination protein 1 (SeV-Myod1), a myogenic master transcription factor. SeV-Myod1 treatment converted human embryonic stem cells (ESCs) into SkM cells, which expressed SkM markers including myosin heavy chain (MHC). We then removed the SeV vector by temporal treatment at a high temperature of 38℃, which also accelerated mesodermal differentiation, and found that SkM cells exhibited fibre-like morphology. Finally, after removal of the residual human ESCs by pluripotent stem cell-targeting delivery of cytotoxic compound, we generated SkM cells with 80% MHC positivity and responsiveness to electrical stimulation. This simple method for myogenic differentiation was applicable to human-induced pluripotent stem cells and will be beneficial for investigations of disease mechanisms and drug discovery in the future. |
記述: | 温度感受性センダイウイルスベクターを用いて ヒトES細胞/iPS細胞から骨格筋細胞を簡便に作製する技術開発 --神経筋疾患病態モデル構築と創薬研究への利用--. 京都大学プレスリリース. 2021-09-13. |
著作権等: | © 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/265438 |
DOI(出版社版): | 10.1111/jcmm.16899 |
PubMed ID: | 34510713 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/210913-160000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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