Downloads: 30

Files in This Item:
File Description SizeFormat 
j.bbrc.2020.04.032.pdf606.29 kBAdobe PDFView/Open
Title: Polyunsaturated fatty acids-enriched lipid from reduced sugar alcohol mannitol by marine yeast Rhodosporidiobolus fluvialis Y2
Authors: Nakata, Shota
Hio, Mamoru
Takase, Ryuichi  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-7423-9407 (unconfirmed)
Kawai, Shigeyuki
Watanabe, Daisuke  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-8831-5765 (unconfirmed)
Hashimoto, Wataru  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0003-0185-2371 (unconfirmed)
Author's alias: 中田, 翔太
日尾, 守
髙瀬, 隆一
渡邉, 大輔
橋本, 渉
Keywords: Marine yeast
Rhodosporidiobolus fluvialis
Lipid synthesis
Polyunsaturated fatty acids
Mannitol
Salt tolerance
Issue Date: Jun-2020
Publisher: Elsevier BV
Journal title: Biochemical and Biophysical Research Communications
Volume: 526
Issue: 4
Start page: 1138
End page: 1142
Abstract: Brown macroalgae is a promising marine biomass for the production of bioethanol and biodiesel fuels. Here we investigate the biochemical processes used by marine oleaginous yeast for assimilating the major carbohydrate found in brown macroalgae. Briefly, yeast Rhodosporidiobolus fluvialis strain Y2 was isolated from seawater and grown in minimal medium containing reduced sugar alcohol mannitol as the sole carbon source with a salinity comparable to seawater. Conditions limiting nitrogen were used to facilitate lipid synthesis. R. fluvialis Y2 yielded 55.1% (w/w) and 39.1% (w/w) of lipids, per dry cell weight, from mannitol in the absence and presence of salinity, respectively. Furthermore, mannitol, as a sugar source, led to an increase in the composition of polyunsaturated fatty acids, linoleic acid (C18:2) and linolenic acid (C18:3), compared to glucose. This suggests that oxidation of mannitol leads to the activation of NADH-dependent fatty acid desaturases in R. fluvialis Y2. Such fatty acid composition may contribute to the cold-flow properties of biodiesel fuels. Our results identified a salt-tolerant oleaginous yeast species with unique metabolic traits, demonstrating a key role as a decomposer in the global carbon cycle through marine ecosystems. This is the first study on mannitol-induced synthesis of lipids enriched with polyunsaturated fatty acids by marine yeast.
Rights: © 2020. This manuscript version is made available under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International 4.0 license.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/266841
DOI(Published Version): 10.1016/j.bbrc.2020.04.032
PubMed ID: 32317185
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks


Export Format: 


This item is licensed under a Creative Commons License Creative Commons