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タイトル: | A versatile and robust cell purification system with an RNA-only circuit composed of microRNA-responsive ON and OFF switches |
著者: | Fujita, Yoshihiko https://orcid.org/0000-0001-6321-2121 (unconfirmed) Hirosawa, Moe Hayashi, Karin Hatani, Takeshi Yoshida, Yoshinori https://orcid.org/0000-0001-5511-9090 (unconfirmed) Yamamoto, Takuya https://orcid.org/0000-0002-0022-3947 (unconfirmed) Saito, Hirohide |
著者名の別形: | 藤田, 祥彦 弘澤, 萌 林, 香倫 羽溪, 健 吉田, 善紀 山本, 拓也 齊藤, 博英 |
発行日: | Jan-2022 |
出版者: | American Association for the Advancement of Science (AAAS) |
誌名: | Science Advances |
巻: | 8 |
号: | 1 |
論文番号: | abj1793 |
抄録: | Human induced pluripotent stem cells (iPSCs) are promising cell resources for cell therapy and drug discovery. However, iPSC-derived differentiated cells are often heterogenous and need purification using a flow cytometer, which has high cost and time consumption for large-scale purification. MicroRNAs (miRNAs) can be used as cell selection markers, because their activity differs between cell types. Here, we show miRNA-responsive ON and OFF switch mRNAs for robust cell purification. The ON switch contains a miRNA-target sequence after the polyadenylate tail, triggering translational activation by sensing the target miRNA. By designing RNA-only circuits with miRNA-ON and -OFF switch mRNAs that encode a lethal ribonuclease, Barnase, and its inhibitor, Barstar, we efficiently purified specific cell types, including human iPSCs and differentiated cardiomyocytes, without flow cytometry. Synthetic mRNA circuits composed of ON and OFF switches provide a safe, versatile, and time-saving method to purify various cell types for biological and clinical applications. |
記述: | 2つの合成mRNAスイッチを活用した純度の高い細胞選別システムの開発. 京都大学プレスリリース. 2022-01-06. Synthetic gene circuits that improve stem cell quality. 京都大学プレスリリース. 2022-01-06. |
著作権等: | Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/267221 |
DOI(出版社版): | 10.1126/sciadv.abj1793 |
PubMed ID: | 34985961 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/220106-040000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/220106-040000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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